Document Type: ORIGINAL RESEARCH PAPER

Authors

Faculty of Marine Science and Technology, Iskenderun Technical University, P.O. Box: 31200 Iskenderun, Hatay, Turkey

Abstract

BACKGROUND AND OBJECTIVES: The eutrophication process is increased by anthropogenic or aquaculture facilities in marine ecosystems. DNA damage biomarkers for fish species detect genotoxic parameters for ecological risk assessment. The aim of the present study was to determine genotoxic potential induced by marine cage culture in Iskenderun Bay on gilthead sea bream (Sparus aurata) using Comet assay. 
METHODS: This study was conducted at cage and reference stations of Iskenderun Bay, Northeastern Mediterranean in January 2017. The wild and cultured samples of S. aurata and water samples were collected from wild and fish farm. 
FINDING: The DNA damages at gill and liver cells of gilthead sea bream in the present study were observed with a higher level of DNA damage in gill cells compared to liver cells, and were determined at the low and minimal scale at the cage and reference stations, respectively. The present study demonstrated that the TP values were recorded at 0.020 and 0.016 mg/L in the cage and reference stations which are at border and below 0.020 mg/L. The DIN values were recorded at 0.097 and 0.075 mg/L in the cage and reference stations, which are at below 0.1 mg/L. The water bodies in the cage and reference stations exhibit Moderate/Mesotrophic water quality The correlations between physical-chemical parameters and DNA damage were shown that DIN, NH4-N, NO3-N and NO2-N in water revealed significant positive correlations with DNA damage levels in gill cells. 
CONCLUSION: The present study provides the first data set on genotoxic damage induced by marine cage culture in Iskenderun Bay on gilthead sea bream. The result of this research is an early warning for the marine system and further detailed research is needed to establish the source of the pollution and monitor environmental pollution. 

Graphical Abstract

Highlights

  • The water bodies in the cage and reference stations in the Iskenderun Bay, North Eastern Mediterranean coastal waters exhibit moderate/mesotrophic water quality;
  • A higher level of DNA damage in the gill cells in comparison to liver cells of S. aurata by Comet assay;
  • The DNA damages at gilthead sea bream were determined at the low and minimal scale at the cage and reference stations from Iskenderun Bay, North Eastern Mediterranean coastal waters;
  • NH3-N, DIN, NH4-N, NO3-N and NO2-N in the water and the DNA damage in gill cells of gilthead sea bream revealed strong contribution to the observed DNA damage.

Keywords

Main Subjects

Anderson, D.Y.T.W.; Yu, T.W.; Phillips, B.J.; Schmezer, P., (1994). The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay.Mutat. Res-Fund. Mol. M., 307(1): 261-271 (11 Pages).

APHA (2005) Standard Methods for the Examination of Water and Wastewater. 21st Edition, American Public Health Association/American Water Works Association/Water Environment Federation, Washington DC.

Arslan, K.; Duman, F.; Kanbur, M.; Akyüz, B., (2016). Genotoxicological Analysis of Naturally Captured and Cultured Rainbow Trout (Oncorhynchus mykiss) in Pınarbasi District of Kayseri, J. Faculty. Vet. Med., Erciyes University, 13(2): 131-138 (8 pages).

Basurco, B.; Lovatelli, A.; García, B., (2011). Chapter 1: Current Status of Sparidae Aquaculture, in: Pavlidis, M.A., Mylonas, C.C. (Eds.), Sparidae: Biology and Aquaculture of Gilthead Sea Bream and Other Species. Blackwell Publishing 366 Ltd., West Sussex, UK. 1-50 (50 pages).

Baudou, F.G.; Ossana, N.A.; Castañé, P.M.; Mastrángelo, M.M.; González Núñez, A.A.; Palacio, M.J.; Ferrari, L., (2019). Use of integrated biomarker indexes for assessing the impact of receiving waters on a native neotropical teleost fish. Sci.  Total Environ., 650(1): 1779-1786 (8 pages).

Butrimavičienė, L.; Baršienė, J.; Greiciūnaitė, J.; Stankevičiūtė, M.; Valskienė, R., (2018). Environmental genotoxicity and risk assessment in the Gulf of Riga (Baltic Sea) using fish, bivalves, and crustaceans. Environ. Sci. Pollut. Res., 25(25): 24818-24828 (11 pages).

Cavalcante, D.G.S.M.; Martinez, C.B.R.; Sofia, S.H., (2008). Genotoxic effects of Roundup® on the fish Prochilodus lineatus. Mutat. Res. Gen. Toxicol. Environ. Mutagen., 655(1-2): 41-46 (6 pages).

Chapman, P.M., (2007). Determining when contamination is pollution—weight of evidence determinations for sediments and effluents. Environ. Int., 33(4): 492-501 (10 pages).

Cho; C.Y.; Hynes, J.D.; Wood, K.R.; Yoshida, H.K., (1991). Quantification of fish culture wastes by biological (nutritional), and chemical (luminological) methods; the development of high nutrient dense (HND) diets. In: Cowey, C.B., Cho, C.Y. (Eds.), Nutritional Strategies and Aquaculture Waste. Proceedings of the First International Symposium on Nutritional Strategies in Management of Aquaculture Waste. University of Guelph, Guelph, Ont., 37–50 (4 pages).

Colin, N.; Porte, C.; Fernandes, D.; Barata, C.; Padrós, F.; Carrassón, M.; Maceda-Veiga, A., (2016). Ecological relevance of biomarkers in monitoring studies of macro-invertebrates and fish in Mediterranean rivers. Sci. Total Environ. 540: 307-323 (17 Pages).

Connon, R.E.; Geist, J.; Werner, I., (2012). Effect-based tools for monitoring and predicting the ecotoxicological effects of chemicals in the aquatic environment. Sensors, 12(9): 12741-12771 (31 pages).

Cripps, S.J.; Bergheim, A., (2000). Solids management and removal for intensive land-based aquaculture production systems. Aquacult. Eng., 22(1-2): 33-56 (24 pages).

De Lapuente, J.; Lourenco, J.; Mendo, S.A.; Borras, M.; Martıns, M.;  Costa, G.P.M.; Pacheco, M., (2015). The Comet Assay and its applications in the field of ecotoxicology: a mature tool that continues to expand its perspectives. Front. Genet., 180: 1-20 (20 pages).

DEA, (2012). Department of Environmental Affairs. (DEA)Chapter 9: Oceans and Coasts: Ocean and Coasts Ecosystem Services Are Important as They Directly and Indirectly Impact on Human Livelihoods, Food Security and Agriculture; Oceans and Coasts, p. 26.

Demir, E.; Turna, F.; Aksakal, S.; Emre, Y.; Emre, N.; Yağcı, A.; Kaya, B., (2015). The comet assay using rainbow trout (Oncorhynchus mykiss) for the detection of nutrient pollution generated from overfed fish farms in the esen stream. Fresenius Environ. Bull., 24(11): 3665-3671 (7 pages).

FAO, (2014). The state of world fisheries and aquaculture. ISBN: 978-92-5-108275-1 Rome, Italy, The Food and Agriculture Organization, p. 243.

FAO, (2018). The state of world fisheries and aquaculture. Rome, Italy. The Food and Agriculture Organization, p. 256.

Gorlach-Lira, K.; Pacheco, C.; Carvalho, L.C.T.; Melo Junior, H.N., Crispm, M.C., (2013). The influence of fish culture in floating net cages on microbial indicators of water quality. Braz. J.  Biol., 73(3): 457-463 (7 pages).

Gürses, R.K.; Büyükateş, Y.; Yiğit, M.; Ergün, S.; Ateş, A.S.; Özdilek, H.G., (2019). Potential Environmental Impacts of Tuna Cage Farming in The Aegean Sea. Aquat. Res., 2(2): 61-72 (12 pages).

Gutiérrez, J. M.; Molisani, M.M.; Da Conceição, M.B.; Weber, L.I., (2019). Characterisation of coastal aquaculture sites in the state of Rio de Janeiro, Brazil, using genotoxicity biomarkers. Reg. Stud.  Mar. Sci., 32: 100870-100876 (7 pages).

Hallare, A.; Ocampo, K.A.; Tayo, P.K., Balolong, M., (2016). Genotoxic stress induced by intensive aquaculture activities in Taal Lake (Philippines) on circulating fish erythrocytes using the comet assay and micronucleus test. Adv. Environ. Biol., 10(1): 273-283 (11 pages).

Kroon, F.; Streten, C.; Harries, S.A., (2017). Protocol for identifying suitable biomarkers to assess fish health: A systematic review. PLOS ONE, 12(4): 1-42 (42 pages).

Lenhardt, M.; Poleksić, V.; Vuković-Gačić, B.; Rašković, B.; Sunjog, K.; Kolarević, S.; Gačić, Z., (2015). Integrated use of different fish related parameters to assess the status of water bodies. Slov. Vet. Res., 52(1): 5-13 (9 pages).

Lı, Z.H.; Zlabek, V.; Velısek, J.; Grabıc, R., Machova, J.; Kolarova, J.; Lı, P.; Randak, T. (2013). Multiple biomarkers responses in juvenile rainbow trout, Oncorhynchus mykis, after acute exposure to a fungicide propiconazole. Environ.Toxicol., 28(3): 119-126 (8 pages).

Mitchelmore, C.L; Chipman, J.K. (1998). DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring. Mutat. Res-Fund. Mol. M., 399(2): 135-147 (13 pages).

Moustafa, Y. T.; Ali, A.; Gomha, S. A.; Mansour, E. M., (2020). Nitrogen and Phosphorus budget for Nile tilapia hatchery. Egypt. J. Aquac., 10(1): 1-22 (22 pages).

Nehls, S.; Segner, H., (2005). Comet Assay with the Fish Cell Line Rainbow Trout Gonad-2 for Invitro Genotoxicity Testing of Xenobiotics and Surface Waters. Environ. Toxicol. Chem., 24(8): 2078-2087 (10 pages).

Olive, P.L., Banáth, J.P., (2006). The comet assay: a method to measure DNA damage in individual cells. Nat. Protoc., 1(1): 23-31 (9 pages).

Ostling, O.; Johanson, K., (1984). Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Bioph. Res. Co., 123(1): 291-298 (8 pages).

Ozbay, G.; Blank, G.; Thunjai, T., (2014). Impacts of aquaculture on habitats and best management practices (BMPs). Sustain. Aquacult. Tech., 1.

Ploetz, D.M.; Fitts, B.E.; Rice, T.M., (2007). Differential accumulation of heavy metals in muscle and liver of a marine fsh (King Mackerel Scomberomorus cavalla Cuvier) from the Northern Gulf of Mexico USA, Bull Environ. Con. Toxicol., 78 (2): 124–127 (4 pages).

Sıngh, N.P.; Mccoy, M.T.; Tıce, R.R.; Schneıder, E.L., (1988). A simple technic for quantitation of low levels of DNA damage in individual cells. Exp. Cell. Res., 175(1): 184-191 (8 pages).

Stephens, W.W.; Farris, J. L., (2004). A biomonitoring approach to aquaculture effluent characterization in channel catfish fingerling production. Aquacult.., 241(1):  319–330 (12 pages).

TEG, (2005). Regulation about the changes in the Aquaculture Regulation announced in Official Gazette No. 25967. Turkish Environmental Guidelines. Ankara. Ministry of Agriculture and Forestry, Turkey.

TEG, (2006). Circular Based on the Regulation of Aquaculture announced in Official Gazette No. 25967. Turkish Environmental Guidelines. Ankara. Ministry of Agriculture and Forestry, Turkey.

TEG , (2012). Surface water quality management regulation. Turkish Environmental Guidelines (in Turkish), Official Gazette (dated 30 November 2012) numbered 28483, Ankara. Publications of Republic of Turkey Ministry of Forestry and Water Affairs.

Tugrul, S.; Ozhan, K.; Akcay, I., (2019). Assessment of trophic status of the Northeastern Mediterranean coastal waters: eutrophication classification tools revisited. Environ. Sci.  Pollut. Res., 26(15): 14742-14754 (13 pages).

Turan, F.; Ergenler, A., (2019). Assessment of DNA Damage by Comet Assay in Trachinotus ovatus Cells from Mersin Bay in the Northeastern Mediterranean, Nat.  Eng. Sci., 4(3): 25-31 (7 pages).

Zheng, X.Q.; Shi, Y.J.; Lu, Y.L.; Xu, X.B., (2016). Growth inhibition and DNA damage in the earthworm (Eisenia fetida) exposed to perfluorooctane sulphonate and perfluorooctanoic acid. Chem. Ecol. 32 (2): 103-116 (14 Pages).

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