Document Type: ORIGINAL RESEARCH PAPER

Authors

1 Ecosystems Research and Development Bureau, Department of Environment and Natural Resources, Los Baños, College, Laguna, 4030 Philippines

2 Mindanao State University at Naawan, Naawan, Misamis Oriental, 9003 Philippines

Abstract

The basic premise of this research was to assess the ethnomedicinal uses of plants in an ancestral forest area at Naawan, Misamis Oriental and determine its inhibition effect against bacterial strains. The assessment of plants was conducted using the transect-plot method. Ethnomedicinal uses and the mode of preparations were obtained using a semi-structured interview questionnaire. Five ethnomedicinal plants: Crinum asiaticum L., Pavetta indica L., Bauhinia purpurea L., Mollugo pentaphylla L., and Cinnamomum mercadoi S. Vidalwere selected for the anti-microbial test against cultured bacterial strains; the Escherichia coli and Staphylococcus aureus using the disc diffusion method. These species are commonly used by the indigenous people and known to cure stomach disorder and wounds. The mode of preparation of the extracts follows the traditional method of the indigenous people. Chloramphenicol, on the other hand, was used as positive control. Thirty-three out of 61 identified species were locally claimed to have medicinal value and are known to cure stomach disorders, poisoning, bleeding, cough, fever and wounds, among others. Antimicrobial test showed greater inhibitory effect of Pavetta indica and Bauhinia purpurea against Staphylococcus aureus and Escherichia coli, correspondingly; however, the synthetic chloramphenicol exhibited greater antibacterial action than any of the plant extracts. In areas where civilization is afar, importance of forest in terms of medicinal uses is highly acknowledged by the local community. Indigenous people, in particular, know the importance of the forest especially in relation to health care system and they believe that there is a need to conserve the resources to sustain the services it provides.

Graphical Abstract

Highlights

  • Thirty one out of sixty-one species assessed in the forest have ethnomedicinal value to the community
  • Chloramphenicol still has the highest inhibitory effect against the two bacterial strains.
  • Among ethnomedicinal plants, Bauhinia purpurea has the highest average zone of inhibition against E. coli while Paveta indica against S. aureus with an average value of 18.50 mm and 15.67 mm, respectively.

Keywords

Main Subjects

Afolayan, A. J., (2003). Extract from the shoots of Arctotis artototdes inhibit the growth of bacteria and fungi. Pharm Biol. (41):22-25 (4 pages).

Alemayehu, G.; Asfaw, Z.;  Kelbessa, E. (2015). Ethnobotanical study of medicinal plants used by local communities of Minjar-Shenkora District, North Shewa Zone of Amhara Region, Ethiopia. Journal of Medicinal Plants Studies; 3(6): 01-11 (11 pages).

Alves, R.R.N.; Rosa, I.L., (2005). Why study the use of animal products in traditional medicines? J. Ethnobiol. Ethnomed., (1): 1-5 (5 pages).

Andrews, W., (1992).  Manuals of food quality control–Microbiological analysis, FAO food and nutrition paper, Food and Agriculture Organization of the United Nations, (338 pages).

Annegowda, H.V.; Mordi, M. N.; Ramanathan, S.; Hamdan, M. R.; Mansor, S. M., (2012). Effect of Extraction Techniques on Phenolic Content, Antioxidant and Antimicrobial Activity of Bauhinia purpurea: HPTLC Determination of Antioxidants. Food Anal. Methods, (5): 226.

Arances, J.; Amoroso, V.B.; Gorne, N.D.; Ruba, R.P.; Rufila, L.V.; Opiso, G.S.; Demetillo, M.T.; Noval, K.G.; Vanden Berg, A.; Stortelder, A.; Kessler, P.J.A.; Gruezo, W. S.M.; Lagunzad, D.A.; Tan, B.C.; Co, L.L.; Aranico, E.C.; Roscom, B.A.; Alava, C.G., (2006). Plant diversity and status in the northern landscape of Mt. Malindang range and environs, misamis occidental, Phillipines. BRP Monograph Series No.8. Biodiversity research program for development in Mindanao: Focus on Mt. Malindang and Environs.SEARCA, College, Laguna.

Asolker, L.V.; Kakkar, K.K.; Chakre, O.J., (2000). Supplement to glossary of Indian medicinal plants. Part-I (A-K). National Institute of Science Communication, New Delhi,, 116–117 (2 pages).

Ayinam, C., (1995). Ecology and ethnomedicine: Exploring links between current environmental crisis and indigenous medical practices. Soc. Sci. Med., 40 (3): 321-329 (9 pages).

Ayyanar, M.; Ignacimuthu, S., (2005). Traditional knowledge of Kani tribals of Kouthalai of Tirunelveli hills, Tamil Nadu, India. J. Ethnopharmacol., 102: 246-255 (10 pages).

Barbour, E.K., Mohammed Al Sharif, Sagherian, A.K., Habre, A.N., Talhouk, R.S., Talhouk, S.N. (2004). Screening of selected indigenous plants of Lebanon for antimicrobial activity. J. Ethnopharmacol., 93: 1–7 (7 pages).

Balick, M.; Cox, P.A., (2003). Plants that heal. In their book Plants, people, and culture: the science of ethnobotany. New York, USA. Sci. Am. Library, 25-61 (37 pages).

Beaglehole, R.; Irwin, A.; Prentice, T., (2004). The World health report 2004, changing history. Statistical Annex. Deaths by cause, sex and mortality stratum in WHO regions, estimates for 2002. WHO, Geneva, 120–122 (3 pages).

Bhalodia, N.R.; Shukla, V.J., (2011). Antibacterial and antifungal activities from leaf extracts of Cassia fistulal.: An ethnomedicinal plant. J. Adv. Pharm. Tech. Res., 2(2): 104–109 (6 pages).

Bletter, N., (2007). A quantitive synthesis of the medicinal ethnobotany of the Malinke of Mali and the Ashaninka of Peru, with the a new theoretical framework. J. Ethnobiol. Ethnomed., 3(36): 1-47 (47 pages).

Cowan, M.M., (1999). Plant products as anti-microbialagents. Clin. Microbiol. Rev.;12: 564–82 (19 pages).

Dahanukar, S.A.; Kulkarni, R.A.; Rege, N.N., (2000). Pharmacology of medicinal plants and natural products. Indian J. Pharmacol., 32: S81–118 (28 pages).

Drew, W.L.; Barry, A.L.; O'toole, R.; Sherris, J.C.M., (1972). Reliability of the kirby-bauer disc diffusion method for detecting methicillin-resistant strains of Staphylococcus aureus. Appl. Microbiol., 24(2):  240-247 (8 pages).

Eloff, J.N., (1988). Which extractant should be used for the screening and isolation of antimicrobial components from plants? J. Ethnopharmacol., 60: 1–8 (8 pages).

Fadeyi, M.O.; Akpan, U.E., (1989). Antimicrobial activities of the leaf extracts of Eugenia uniflora LINN (Synonm Stenocalyx Michelli LINN) myrtaceae. Phototherapy Res. J., (3): 154.  (2 pages).

Geetha R.V.; Roy, A.; Lakshmi, T., (2011). Nature’s Weapon Against Urinary Tract Infections. Int. J. Drug Dev. Res., 3(3): 85-100 (16 pages).

Golwala D.K.; Patel L.D.; Bothara S.B.; Patel P.M.; Vaidya S.K.; Raval, M.K., (2009). Analgesic activity of ethanolic leaf extract of Pavetta indica. Int. J. Pharm. Sci. Drug Res., (1): 119-120 (2 pages).

Guerrero, L.M., (1921). Medicinal uses of Philippines plants. Philippines Bur. Forestry Bull., 22: 149-246 (97 pages).

Heinrich, M.; Barnes, J.; Gibbons, S.; Williamson, E.M., (2004). Fundamentals of pharmacognosy and phytotherapy. Churchill Livingstone, Edinbrugh, 245–252 (8 pages).

IUCN, (2011). IUCN Red list of threatened species. Version 2011.1.

Jeruto, P.; Lukhoba, C.; Ouma, C.; Otieno, G.; Mutai, C., (2008). An ethnobotanical study of medicinal plants used by the Nandi people in Kenya. Journal of Ethnopharmacology 116, 370–376 (7 pages).

Kirthikar, K.R.; Basu, D.B., (2001). Indian medicinal plants, vol. 4, 2nd edn. Oriental Enterprises, Dehradun, 1255–1257 (3 pages).

Kumar, A. (2006). Sri Lakshmi Narasimha College of Pharmacy India. The 9th International Congress on Ethnopharmacology NICE.

Lulekal, E.; Asfaw, Z.; Kelbessa, E.; Damme, P.V., (2013). Ethnomedicinal study of plants used for human ailments in Ankober District, North Shewa Zone, Amhara Region, Ethiopia. J. Ethnobiol. Ethnomed., 9(63): 1-13 (13 pages).

Madulid, D.A., (2001). A dictionary of Philippine plant names. Bookmark, Inc. 1: 781 (781 pages).

Mandal S. C.; Lakshmi S. M.; Kumar C. K. A.; Sur T. K.; Boominathan R., (2003). Evaluation of anti-inflammatory potential of Pavetta indica Linn. leaf extract (family: Rubiaceae) in rats. Phytother Res. (17): 817-820 (4 pages).

Mason, T.C.; Wasserman, B.P., (1987).  Inactivation of red beet betaglucan synthase by native and oxidized phenolics compounds. Phytochem., (26): 2197-2202 (6 pages).

McGregor, D.H.; Chu, L.L.; MacGregor, R.R. ; Cohn, D.V., (1997). Disruption of the Golgi zone and inhibition of the conversion of proparathyroid hormone to parathyroid hormone in human parathyroid tissue by tris (hydroxymethyl) aminomethane. Am. J. Pathol., 87(3): 553–568 (16 pages).

Murugan, M.; Mohan, V.R., (2011). Evaluation of phytochemical analysis and antibacterial activity of Bauhinia purpurea L. and Hiptage benghalensis L. Kurz. J. Appl. Pharm. Sci., 01 (09): 157-160 (4 pages).

Nikaido, H.; Vaara, M., (1985). Molecular basis of bacterial outer membrane permeability. Microbiol. Rev.  49: 1-32 (32 pages).

Owolabi, J.; Omogbai, E.K.I.; Obasuyi, O., (2007). Antifungal and antibacterial activities of the ethanolic and aqueous extract of Kigella africana (Bignoniaceae) stem bark. Afr.J. Biotechnol., 6: 882-885 (4 pages).

Penna, C.; Marino, S.; Vivot, E.; Cruanes, M.C.; de D Munoz, J.D.; Cruanes, J.; Ferraro, G.; Gutkind, G.; Martino, V., (2001). Antimicrobial activity of Argentine plants used in the treatment of infectious diseases: isolation of active compounds from Sebastiania brasiliensis. J Ethnopharmacol., (77): 37–40 (4 pages).

Prasuna, M.L.; Chandel, U., (2015). Studies of antibacterial activities of leaf extract of selected etheno –medicinal plants. IOSR J. Environ. Sci. Toxicol. Food Tech., 1(3): 65-66 (2 pages).

Quisumbing, E., (1978). Medicinal plants of the phils. Katha Publishing Co., Inc. 321-322 (2 pages).

Ren, W.; Qiao, Z.; Wang, H.; Zhu, L.; Zhang, L., (2003). Flavonoids: promising anticancer agents. Med. Res. Rev., (23): 519–534 (16 pages).

Rios, J.L.; Recio, M.C., (2005). Medicinal plants and antimicrobial activity. J. Ethnopharmacol., 100: 80–84 (5 pages).

Roy, A.; Gupta, V.K.; Kaur, C.; Simlai, A., (2013). Antimicrobial activity of pavetta indica leaves. J. Appl. Pharm. Sci., 3(4): 078-082. (4 pages).

Rojo, J.P., (1999). Revised lexicon of Philippine trees. Forest Products Research and Development Institute. Department of Science and Technology, (484 pages).

Sarac, N.; Ugur, A., (2007). Antimicrobial activities and usage in folkloric medicine of some Lamiaceae species growing in Mugla, Turkey. Eur. Asia J. Bio. Sci., (4): 28-37 (9 pages).

Selvamohan, T.; Ramadas, V.; Shibila Selva Kishore, S., (2012). Antimicrobial activity of selected medicinal plants against some selected human pathogenic bacteria. Adv. Appl. Sci. Res., 3 (5): 3374-3381 (8 pages).

Shaikh, D.; Malika, F.A.; Rafi, S.M.; Naqui B., (1994). Studies of antibacterial activity of ethanolic extract from Nericum indicum and Hibiscus rosasinensis. J. Islamic Acad Sci., 7: 167–168 (2 pages).

Shreedhara, C.S.; Vaidya, V.P.; Vagdevi, H.M.; Latha, K.P.; Muralikrishna, K.S.; Krupanidhi, A.M., (2009). Screening of Bauhinia purpurea Linn. for analgesic and anti-inflammatory activities. Indian J. Pharmacol., 41 (2): 75–79 (5 pages).

Tambekar, D.H.; Dahikar, S.B., (2011). Exploring antibacterial potential of some Ayurvedic preparations to control bacterial enteric infection. J. Chem. Pharm. Res. 5:494-501 (8 pages).

Thabrew M. I.; Joice P. D.; Rajatissa W., (1987). A comparative study of the efficacy of Pavetta indica and Osbeckia octanda in the treatment of liver dysfunction. Planta Med. (53): 239-241 (3 pages).

Torres, R.C.; Sison, F.M.; Ysrael, M.C., (2003). Phytochemical screening and biological studies on the Crude Methanol Extract of Cinnamomum Mercadoi, Vidal. Philippine J. Sci., 132 (1): 27-32 (6 pages).

Tsuchiya, H.; Sato, M.; Miyazaki J.; Fujiwara, S.; Tanigaki, S.; Ohyama, M.; Tanaka, T.; Inuma, M., (1996). Comparative study on the antibacterial activity of phytochemical flavanones against methicillin - resistant Staphylococcus aureus. J. Ethnopharmacol., (50): 27-34 (8 pages).

WHO, (1993). Summary of WHO guidelines for the assessment of herbal medicines. World Health Organization, Herbal Gram. 28: 13-14 (2 pages).

Ya, C.; Craffiney, S.H.; Lilley, T.H.; Hartam, E., (1988). Carbohydrate polyphenol complexation. In: chemistry and significance of condensed tannins. Hemingway, R.W. and kenchesy, J.J. (Eds). Plenum press, New York, (553 pages).

Zakaria, Z.A.; Wen, L.Y.; Abdul Rahman, N.I.; Abdul Halim, A.A.; Sulaiman, M.R.; Gopalan, H.K., (2007). Antinociceptive, anti-inflammatory and antipyretic properties of the aqueous extract of Bauhinia purpurea leaves in experimental animals. Med. Princ. Pract., (16): 443–449 (7 pages).

 


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