Department of Civil Engineering, Vel Tech High Tech Dr.Rangarajan Dr.Sakunthala Engineering College, Avadi, Chennai 600062, Tamil Nadu, India


Taguchi L9 orthogonal array was implemented to select optimum values of process parameters and to attain the maximum removal of pollutants and power generation from dairy industry wastewater using double chambered salt bridge microbial fuel cell. The maximum chemical oxygen demand reduction, current, voltage, power, current density and power density in double chambered salt bridge microbial fuel cell from dairy industry wastewater was found to be 86.30 %, 16.10 mA, 886.34 mV, 14.27 mW, 1219.69 mA/m2 and 1081.06 mW/m2 respectively for the optimum value of 1M NaCl concentration, 10 % agar concentration and 0.10 m salt bridge length. Double chambered salt bridge microbial fuel cell was not only removed chemical oxygen demand and produced power, but it also removed other pollutants at the maximum level against the best optimum value of process parameters from the dairy industry wastewater. The proposed regression model was used to select the right combination of process parameters for obtaining a maximum reduction of pollutants and simultaneous power production from the dairy industry wastewater.

Graphical Abstract

Pollution reduction and electricity production from dairy industry wastewater with microbial fuel cell


  • Ferrite electrode used in anode and cathode chambers enhances DCSB-MFC performances towards bioelectricity production;
  • The maximum COD reduction and power production in DCSB-MFC from dairy industry wastewater were found to be 86.30 % and 14.27 mW, respectively;
  • Improvement in power production and pollutant reduction was observed with 1M NaCl concentration, 10% agar concentration and 0.05 m salt bridge length through the Taguchi optimization approach;
  • A model simulation was framed to select the best combination of process parameters towards the targeted maximum power production and pollutants reduction from dairy industry wastewater using DCSB-MFC.


Adeleye, S.A.; Okorondu, S.I., (2015). Bioelectricity from students’ hostel waste water using microbial fuel cell. Int. J. Biol. Chem. Sci., 9(2): 1038–1049 (12 pages).

Akshay, D.T.; Namrata, S.; Osborne, W.J., (2016). Microbial fuel cells in bioelectricity production. Front. Life Sci., 9(4): 252–266 (15 pages).

Ali, A.H.; Al-Mussawy, H.A.; Hussein, M.J.; Hamadi, N.J., (2018). Experimental and theoretical study on the ability of microbial fuel cell for electricity generation. Pollution, 4(2): 359–368 (10 pages).

APHA, (2017). Standard methods for the examination of water and wastewater, 23rd ed. American Public Health Association; American Water Works Association; Water environment federation, Washington DC, USA.

Boas, J.V.; Oliveira, V.B.; Marcon, L.R.C.; Pinto, D.P.; Simoes, M.; Pinto, A.M.F.R., (2015). Effect of operating and design parameters on the performance of a microbial fuel cell with Lactobacillus pentosus. Biochem. Eng. J., 104: 34–40 (7 pages).

Dewan, A.; Donovan, C.; Heo, D.; Beyenal, H., (2010). Evaluating the performance of microbial fuel cells powering electronic devices. J. Power Sources, 195(1): 90–96 (7 pages).

Drisya, C.M.; Manjunath, N.T., (2017a). Dairy wastewater treatment and electricity generation using microbial fuel cell. Int. Res. J. Eng. Technol., 4(8): 1293–1296 (4 pages).

Drisya, C.M.; Manjunath, N.T., (2017b). Impact of nanoparticle incorporated salt bridge on bioelectricity production and treatment efficiency of microbial fuel cell. Int. J. Sci. Res. Dev., 5(6), 2104–2107 (4 pages).

Feng, Y.; Barr, W.; Harper Jr, W.F., (2013). Neural network processing of microbial fuel cell signals for the identification of chemicals present in water. J. Environ. Manage., 120: 84–92 (9 pages).

Fradler, K.R.; Kim, J.R.; Boghani, H.C.; Dinsdale, R.M.; Guwy, A.J.; Premier, G.C., (2014). The effect of internal capacitance on power quality and energy efficiency in a tubular microbial fuel cell. Process Biochem., 49: 973–980 (8 pages).

Ghasemi, M.; Wan Daud, W.R.; Alam, J.; Ilbeygi, H.; Sedighi, M.; Ismail, A.F.; Yazdi, M.H.; Aljlil, S.A., (2016). Treatment of two different water resources in desalination and microbial fuel cell processes by poly sulfone/sulfonated polyether ether ketone hybrid membrane. Energy, 96: 303–313 (11 pages).

Jafary, T.; Aljlil, S.A.; Alam, J.; Ghasemi, M., (2017).  Effect of the membrane type and resistance load on the performance of the microbial fuel cell: A step ahead of microbial desalination cell establishment. J. Japan Inst. Energy, 96(9): 346–351 (6 pages).

Khare, A.P., (2014). Voltage produced by different salts concentration on single chamber microbial fuel cell. Int. J. Eng. Sci. Res. Technol., 3(3): 1448–1452 (5 pages).

Li, W.W.; Yu, H.Q.; He, Z., (2014). Towards sustainable wastewater treatment by using microbial fuel cells centered technologies. Energy Environ. Sci., 7(3): 911–924 (14 pages).

Li, X.M.; Cheng, K.Y.; Selvam, A.; Wong, J.W.C., (2013a). Bioelectricity production from acidic food waste leachate using microbial fuel cells: Effect of microbial inocula. Process Biochem., 48: 283–288 (6 pages).

Li, X.M.; Cheng, K.Y.; Wong, J.W.C., (2013b). Bioelectricity production from food waste leachate using microbial fuel cells: Effect of NaCl and pH.  Bioresour. Technol., 149: 452–458 (7 pages).

Liu, W.F.; Cheng, S.A., (2014). Microbial fuel cells for energy production from wastewaters: the way toward practical application. J. Zhejiang Univ. Sci. A (App. Phys. Eng.), 15(11): 841–861 (21 pages).

Logan, B.E., (2010). Scaling up microbial fuel cells and other bioelectro chemical systems. Appl. Microbiol. Biotechnol., 85(6): 1665–1671 (7 pages).

Logan, B.E.; Hamelers, B.; Rozendal, R.; Schroder, U.; Keller, J.; Freguia, S.; Aelterman, P.; Verstraete, W.; Rabaey, K., (2006). Microbial fuel cells: methodology and technology. Environ. Sci. Technol., 40(17): 5181–5192 (12 pages).

Moqsud, A.M.; Omine, K.; Yasufuku, N.; Hyodo, M.; Nakata, Y., (2013). Microbial fuel cell (MFC) for bioelectricity generation from organic wastes. Waste Manage., 33: 2465–2469 (4 pages).

Oliveira, V.B.; Simões, M.; Melo, L.F.; Pinto, A.M.F.R., (2013). Overview on the developments of microbial fuel cells. Biochem. Eng. J., 73: 53–64 (12 pages).

Pant, D.; Bogart, G.V.; Carrero, C.P.; Diels, L.; Vanbroekhoven, K., (2010). A review of the substrates used in microbial fuel cells (MFCs) for sustainable energy production. Biores. Technol. 101: 1533–1543 (11 pages).

Parkash, A.; Aziz, S.; Nazir, I.; Soomro, S.A., (2015). Utilizing dairy wastewater for electricity generation using environment-friendly double chambered microbial fuel cell. NUST J. Eng. Sci., 8(1): 44–50 (7 pages).

Rahimnejad, M.; Ghasemi, M.; Najafpour, G.D.; Ismail, M.; Mohammad, A.W.; Ghoreyshi, A.A.; Hassan, S.H.A., (2012). Synthesis, characterization and application studies of self-made Fe3O4/PES nanocomposite membranes in microbial fuel cell. Electrochim. Acta, 85: 700–706 (7 pages).

Sahana, M.S.; Manjunath, N.T., (2018). Performance evaluation of MFC in treating dairy wastewater and electricity generation using zinc and copper electrodes. Int. J. Adv. Res. Eng. Technol., 6(7): 22–25 (4 pages). 

Sarma, D.; Thakuria, M.; Dey, N.; Nath, S.; Barua, P.B.; Mallick, S., (2019). Investigation and Taguchi optimization of microbial fuel cell salt bridge dimensional parameters. J. Inst. Eng. India Ser. C, 100(1): 103–112 (10 pages).

Sedighi, M.; Aljlil, S.A.; Alsubei, A.D.; Ghasemi, M.; Mohammadi, M., (2018). Performance optimisation of microbial fuel cell for wastewater treatment and sustainable clean energy generation using response surface methodology. Alexandria Eng. J., 57: 4243–4253 (11 pages).

Sevda, S.; Sreekrishnan, T.R., (2012). Effect of salt concentration and mediators in salt bridge microbial fuel cell for electricity generation from synthetic wastewater. J. Environ. Sci. Health, Part A, 47(6): 878–886 (9 pages).

Shahgaldi, S.; Ghasemi, M.; Wan Daud, W.R.; Yaakob, Z.; Sedighi, M.; Alam, J.; Ismail, A.F., (2014). Performance enhancement of microbial fuel cell by PVDF/Nafion nanofibre composite proton exchange membrane. Fuel Process. Technol., 124: 290–295 (6 pages).

Sivakumar, D., (2015). Hexavalent chromium removal in a tannery industry wastewater using rice husk silica.  Global J. Environ. Sci. Manage., 1(1): 27–40 (14 pages).

Sivakumar, D., (2016). Biosorption of hexavalent chromium in a tannery industry wastewater using fungi species. Global J. Environ. Sci. Manage. 2(2): 105–124 (20 pages).

Venkata Mohan, S.; Roghavalu, S.; Srikanth, G.; Sarma, P.N., (2007). Bioelectricity production by mediator less microbial fuel cells under acidophilic conditions using wastewater as substrate loading rate. Current Sci., 92(12): 1720–1726 (7 pages).

Venkata Mohan, S.; Veer Raghavulu, S.; Dinakar, P.; Sarma, P.N., (2009a). Integrated function of microbial fuel cell (MFC) as bio-electrochemical treatment system associated with bioelectricity generation under higher substrate load, Biosens. Bioelectron., 24: 2021–2027 (7 pages).

Venkata Mohan, S.; Veer Raghavulu, S.; Mohanakrishna, G.; Srikanth, S.; Sarma, P.N., (2009b). Optimization and evaluation of fermentative hydrogen production and wastewater treatment processes using data enveloping analysis (DEA) and Taguchi design of experimental (DOE) methodology. Int. J. Hydrogen Energy, 34: 216–226 (11 pages).

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