Document Type: ORIGINAL RESEARCH PAPER

Authors

1 Department of Environmental Engineering, Universitas Pembangunan Nasional Veteran Jawa Timur, Raya Rungkut Madya Gunung Anyar Surabaya, Indonesia

2 Graduate School of Environmental Management, Tajen University, Weixin Road, Yanpu Township, Pingtung Country, Taiwan

Abstract

A combination of high performance size exclusion chromatography with organic carbon detector and ultraviolet detector coupled with peak-fitting technique and fluorescence excitation-emission matrix spectrometry applied fluorescence regional integration method was conducted to determine the characteristics of organic matter during nitrification. The batch scale of bionet nitrification without organic carbon substrate under aerobic conditions was operated for around 150 minutes. Bulk organic parameters and NH4+-N concentration were analyzed. Five different molecular weights of organic matter were identified by using chromatography, and five different groups of fluorophores organic fractions detected by fluorescence. According to chromatography with carbon and ultraviolet detector, the main characteristics of organic matter shifted from building blocks aromatic compounds with percentage peak area of carbon/ultraviolet detector: 31%/53% to 14%/27.5% to humic-like substances with percentage peak area of carbon/ultraviolet detector 21%/17% to 27%/46.5% during nitrification. Those former compounds are biodegradable as well as properties of microbial products released during substrate utilization and endogenous phase, which are mainly identified as humic-like substances, thus underwent further biodegradation. However, there was significant change in the fluorophores organic fractions, which exhibited humic acid-like with percentage fluorescence regional index area 53% into 68%, as shown by fluorescence excitation-emission matrix analysis. A combination of these methods indicated that the organic matter released during nitrification mainly consists of humic compounds. These results conjecture that a combination of high performance size exclusion chromatography with carbon and ultraviolet detector and fluorescence excitation-emission matrix can be used to determine the characteristic of organic matter and water quality change during nitrification.

Graphical Abstract

Highlights

  • Combination of HPSEC-OCD/UVD and FEEM can be used to determine the characteristic of organic matter and water quality change during nitrification;
  • During nitrification, the major component of aromatic organic matter changed from low molecular weight of building blocks into high molecular weight of humic substances;
  • Simultaneous removing the NH4+-N concentration and increasing organic carbon at the same time revealed microbial products also released, mainly humic substances fraction.

Keywords

Main Subjects

APHA, (2005). Standard methods for the examination of water and wastewaters, 21th edition, APHA, AWWA, WEF Publication, Washington D.C.

Barker, D.J.; Tuckey, D.C., (1999). A review of soluble microbial products (smp) in wastewater treatment systems. Water Res., 30(14): 3063-3082 (20 pages).

Chang, J.J.; Mei, J.; Jia, W.; Chen, J.Q.; Li, X.; Ji, B.H.; Wu, H.M., (2019). Treatment of heavily polluted river water by tidal-operated biofilters with organic/inorganic media: evaluation of performance and bacterial community. Biores. Technol., 279: 34-42 (9 pages).

Chen, W.; Westerhoff, P.; Leenheer, J.A.; Booksh, K., (2003). Fluorescence excitation-emission matrix regional integration to quantify spectra for dissolved organic matter. Environ. Sci. Technol., 37(24): 5701-5710 (10 pages).

Chow, C. W. K.; Fabris, R.; van Leeuwen, J.; Wang, D. S.; Drikas, M., (2008). Assessing natural organic matter treatability using high performance size exclusion chromatography. Environ. Sci. Technol., 42: 6683-6689 (7 pages).

Edzwald, J.K.; Tobiason, J.E., (2011). Chemical principles, source water composition, and watershed protection. Water quality and treatment: a handbook on drinking water, AWWA, McGraw-Hill, New York.

Greenstein, K.E.; Lew, J.; Dickenson, E.R.V.; Wert, E.C., (2018). Invstigation of biotransformation, sorption, and desorption of multiple chemical contaminants in pilot-scale drinking water biofilters. Chemosphere. 200: 248-256 (9 pages).

Guo, L.; Lu, M.M.; Li, Q.Q.; Zhang, J.W.; She, Z.L., (2015). A Comparison of different pretreatments on hydrogen fermentation from waste sludge by fluorescence excitation-emission matrix with regional integration analysis. Int. J. Hydrogen Energy. 40 (1): 197-208 (12 pages).

Han, Z.Y.; Liu, D.; Li, Q.B., (2013). A removal mechanism for organics and nitrogen in treating leachate using a semi-aerobic aged refuse biofilter. J. Environ. Manage., 114: 336-342 (7 pages).

Hidayah, E.N.; Chen, Y.C.; Yeh, H.H., (2017). Comparison between HPSEC-OCD and F-EEMs for assessing DBPs formation in water. J. Environ. Sci. Health. Part A., 52(4): 391-402 (12 pages).

Hidayah, E.N.; Cahyonugroho, O.H., (2019). Tracking of dissolved effluent organic matter (dEfOM) in wastewater treatment plant by using fluorescence method. IOP Conference Series: Earth and Environmental Science, 245(1).

Ho, H.J.; Cao, J.W.; Kao, C.M.; Lai, W.L., (2019). Characterization of released metabolic organic during AOC analyses by P17 and NOX strains using 3-D fluorescent signals. Chemosphere. 222: 205-213 (9 pages).

Huber, S.A.; Balz, A.; Abert, M.; Pronk, W., (2011). Characterisation of aquatic humic and non-humic matter with size-exclusion chromatography-organic carbon detection- organic nitrogen detection (LC-OCD-OND). Water Res., 45: 879-885 (7 pages).

Jacome, A.; Molina, J.; Novoa, R.; Suarez, J.; Ferreiro, S., (2013). Simultaneous carbon and nitrogen removal from municipal wastewater in full-scale unaerated/aerated submerged filters. Water Sci. Technol., 69 (1): 217-221 (5 pages).

Jiao, R.Y.; Chow, C.W.K.; Xu, H.; Yang, X.F.; Wang, D.S., (2014). Organic removal assessment at full-scale treatment facilities using advanced organic characterization tools. Environ. Sci. Processes Impacts, 16: 2451-2459 (9 pages).

Kim, H.C.; Dempsey, B.A., (2012). Comparison of two fractionation strategies for characterization of wastewater effluent organic matter and diagnosis of membrane fouling. Water Res., 46: 3714-3722 (9 pages).

Krasner, S.W.; Mitch, W.A.; McCurry, D.L.; Hanigan, D.; Westerhoff, P., (2013). Formation, precursors, control, and occurrence of nitrosamines in drinking water: A review. Water Res., 47 (13): 4433-4450 (18 pages).

Lai, W.L.; Chen, L.F.; Liao, S.W.; Hsu, S.L.; Tseng, L.H.; Miaw, C.L., (2007). Using EEFM (excitation emission fluorescence matrix) to differentiate the organic properties of the effluents from the ozonated biofilters. Water Air Soil. Pollut., 186(1-4): 43-53 (11 pages).

Lai, C.H.; Chou, Y.C.; Yeh, H.H., (2015). Assessing the interaction effects of coagulation pretreatment and membrane material on UF fouling control using HPSEC combined with peak-fitting. J. Membrane Sci., 474: 207-214 (8 pages).

Laspidou, C.S.; Rittmann, B.E., (2002). A unified theory for extracellular polymeric substances, soluble microbial products, and active and inert biomass. Water Res., 36: 2711-2720 (10 pages).

Li, W.T.; Xu, Z.X.; Li, A.M.; Wu, W.; Zhou, Q.; Wang, J.N., (2013). HPLC/HPSEC-FLD with multi-excitation/emission scan for EEM interpretation and dissolved organic matter analysis. Water Res., 47 93): 1246-1256 (11 pages).

Liu, Y.W., (2013). Pilot-study on nitrification performance of bionet for ammonia removal in raw water of drinking water treatment. Master Thesis. Department of Environmental Engineering, National Cheng University, Taiwan (105 pages).

Liu, J.L.; Li, X.Y.; Xie, Y.F.; Tang, H., (2014). Characterization of soluble microbial products as precursors of disinfection byproducts in drinking water supply. Sci. Total Environ., 472: 8818-8824 (7 pages).

Liu, Y.; Sun, J.; Peng, L.; Wang, D.; Dai, X.; Ni, B.J., (2016). Assessment of heterotrophic growth supported by soluble microbial products in anammox biofilm using multidimensional modeling. Sci Rep., 6:27576 (11 pages).

Matilainen, A.; Gjessing, E.T.; Lahtinen, T.; Hed, L.; Bhatnagar, A.; Sillanpaa, M., (2011). An overview of the methods used in the characterization of natural organic matter (NOM) in relation to drinking water treatment. Chemosphere. 83 (11): 1431-1442 (12 pages).

Matsumoto, S.; Kotaku, M.; Saeki, G.; Terada, A.; Aoi, Y.; Tsuneda, S.; Picioreanu, C.; van Loosdrecht, M.C.M., (2010). Microbial community structure in autotrophic nitrifying granules characterized by experimental and simulation analyses. Environ. Microbiol. 12(1):192-206 (15 pages).

Moradi, S.; Liu, S.; Chow, C.W.K.; van Leeuwen, J.; Cook, D.; Drikas, M; Amal, R., (2017). Developing a chloramine decay index to understand nitrification: a case study of two chloraminated drinking water distribution systems. J. Environ. Sci., 57: 170-179 (10 pages).

Moradi, S.; Sawade, E.; Aryal, R.; Chow, C.W.K.; van Leeuwen, J.; Drikas, M.; Cook, D.; Amal, R., (2018). Tracking changes in organic matter during nitrification using fluorescence excitation-emission matrix spectroscopy coupled with parallel factor analysis (feem/parafac). J. Environ. Chem. Eng., 6(1): 1522-1528 (7 pages).

Murphy, K.R.; Stedmon, C.A.; Graeber, D.; Bro, R., (2013). Fluorescence spectroscopy and multi-way techniques. PARAFAC. Anal. Methods, 5(23): 6557-6566 (10 pages).

Ni, B.J.; Zeng, R.J.; Fang, F.; Xie, W.M.; Sheng, G.P.; Yu, H.Q., (2010). Fractionating soluble microbial products in the activated sludge process. Water Res., 44: 2292-2302 (11 pages).

Ni, B.J.; Xie, W.M.; Chen, Y.P.; Fang, F.; Liu, S.Y.; Ren, T.T.; Sheng, G.P.; Yu, H.Q.; Liu, G.; Tian, Y.C., (2011). Heterotrophs grown on the soluble microbial products (SMP) released by autotrophs are responsible for the nitrogen loss in nitrifying granular sludge. Biotechnol. Bioeng., 108(12): 2844-2852 (9 pages).

Shon, H.K.; Vigneswaran, S.; Snyder, S.A., (2012). Effluent organic matter (EfOM) in wastewater: constituents, effect, and treatment. Crit. Rev. Environ. Sci. Technol., 36(4): 327-374 (48 pages).

Tian, Y.; Chen, L.; Jiang, T.L., (2011). Characterization and modeling of the soluble microbial products in membrane bioreactor. Sep. Purif. Technol., 70: 316-324 (9 pages).

Urbain, V.; Mobarry, B.; de Silva, V.; Stahl, D. A.; Rittmann, B. E.; Manem, J., (1998). Integration of performance, molecular biology and modeling to describe the activated sludge process. Water Sci. Technol., 37: 223-229 (7 pages).

Zeng, T.; Mitch, W.A., (2016). Impact of nitrification on the formation of N-nitrosamines and halogenated disinfection byproducts within distribution system storage facilities. Environ. Sci. Technol., 50 (6): 2964-2973 (10 pages).

Xie, W.M.; Ni, B.J.; Sheng, G.P.; Seviour, T.; Yu, H.Q., (2016). Quantification and kinetic characterization of soluble microbial products from municipal wastewater treatment plants. Water Res., 88: 703-710 (8 pages).

Zhiji, D.; Bourven, I.; van Hullebusch; Panico, A.; Pirozzi, F.; Esposito, G.; Guibaud, G., (2017). Quantitative and qualitative characterization of extracellular polymeric substances from Anammox enrichment. J. Taiwan Inst. Chem. Eng., 80: 738-746 (9 pages).


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