A novel microbial consortium from sheep compost for decolorization and degradation of Congo red

Abd El-Kader, Sh.F.; El-Chaghaby, G.A.; Khalafalla, G.M.; Refae, R.I.; Elshishtawy, H.M.

doi:10.22034/gjesm.2019.01.05

Document Type : ORIGINAL RESEARCH ARTICLE

Authors

¹ Regional Center for Food and Feed, Agricultural Research Center, Cairo, Egypt

² Microbiology Department, Faculty of Agriculture, Cairo University, Cairo, Egypt

³ Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Cairo, Egypt

https://doi.org/10.22034/gjesm.2019.01.05

Abstract

Congo red is a synthetic azo-dye dye with many industrial applications. The effluents containing azo dyes are causing several environmental hazards and thus should be treated prior to their discharge. The present work investigates the possible use of a novel microbial consortium from sheep compost for the decolorization of Congo red dye. The effect of different parameters including contact time, dye concentration and inoculum concentration on dye decolorization were investigated. The kinetic of dye decolorization was also assessed and the biodegradation of the dye was confirmed by different techniques. The results showed that the microbial consortium decolorized about 98% of Congo red (500 mg/L) after 24h. The efficiency of the decolorization decreased from 95% to 62% when the dye concentration increased from 100 to 500mg/L. Also, it was noticed that 75% of Congo red (25 mg/L) was decolorized at an inoculum rate of 2.5%. The kinetic results suggested that the decolorization of Congo red by the studied consortium follows the first order kinetic model. Also the maximum substrate consumption rate (V_max) according to Michaelis- Menten model was found to be 19.30 mg/h/L and the decolorization rate constant (K_m) was 116.93 mg/L. The biodegradation of Congo red was further confirmed by HPLC and GC-Ms analysis which revealed the presence of some spectral differences between the untreated dye sample and the treated one. In conclusion, the results of the present work suggest that microbial consortium from sheep compost could have potential application for bioremediation of industrial effluents containing Congo red dye.

Graphical Abstract

Highlights

Sheep farm compost was used as novel source of microbial consortium
Microbial consortium successfully decolorized Congo red solutions
Removal efficiency of dye reached 98% after 24 hours
Small amount of consortium (2.5%) decolorized more than 75% of Congo red.

Keywords

References

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Global Journal of Environmental Science and Management

A novel microbial consortium from sheep compost for decolorization and degradation of Congo red

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Volume 5, Issue 1 - Serial Number 17
January 2019
Pages 61-70

A novel microbial consortium from sheep compost for decolorization and degradation of Congo red

References

References

Letters to Editor

Send comment about this article

Volume 5, Issue 1 - Serial Number 17January 2019Pages 61-70

Volume 5, Issue 1 - Serial Number 17
January 2019
Pages 61-70