1 Department of Civil Engineering, Vel Tech High Tech Dr.Rangarajan Dr.Sakunthala Engineering College, Avadi, Chennai 600062, Tamil Nadu, India

2 School of Environmental Health Engineering, Tehran University of Medical Sciences, Tehran, Iran


This study was focused on removal of various parameters in paper mill effluent using a method called bioremediation by Azolla caroliniana.  The experimental investigations have been carried out using Azolla caroliniana for conducting the sorption study with various dilution ratios (2, 4, 6, 8, and 10), pH (3, 4, 5, 6, 7, 8 and 9) and biomass (200, 400, 600, 800 and 1000 g). The maximum removal percentage of TDS, BOD and COD in a paper mill effluent was obtained at the optimum dilution ratio of 6, pH of 8 and biomass of 800 g. The results of this study indicated that the maximum removal percentage of TDS, BOD and COD in a paper mill effluent was 82.3 %, 88.6 % and 79.1 % respectively.  Also, the study focused on uptake of TDS, BOD and COD in paper mill effluent by Azolla caroliniana through bioaccumulation factor and translocation factor. The results of bioaccumulation factor revealed that TDS, BOD and COD in paper mill effluent were adsorbed by Azolla caroliniana.  The results of translocation factor revealed that the roots of Azolla caroliniana translocate the TDS, BOD and COD in a paper mill effluent to the shoots of Azolla caroliniana. From the results, this study concluded that bioremediation by Azolla caroliniana could be effectively used for removing TDS, BOD and COD in a paper mill effluent. This study also suggested that Azolla caroliniana may be used for removing various contaminants, not only from paper mill effluent, but also from any other industrial effluents.


Akbal, F.; Camc, S., (2010). Comparison of electrocoagulation and chemical coagulation for heavy metal removal. Chem. Eng. Tech., 33: 1655–1664 (10 pages).

APPA, AWWA, and WEF, (2005), Standard methods for the examination of water and wastewater, 20th edition, APHA Publication, Washington D.C.

Bellebia, S. Kacha, S. Bouyakoub, A.Z. Derriche, Z. (20120. Experimental investigation of chemical oxygen demand and turbidity removal from cardboard paper mill effluents using combined electrocoagulation and adsorption processes, Environ. Prog. Sustain. Energy, 31: 361–370 (10 pages).

Chen Chen, Shengtao Mao, Jingjing Wang, Jinfeng Bao, Hui Xu, Wenpeng Su, and Hongqi Dai, (2015). Application of ultrafiltration in a paper mill: Process water reuse and membrane fouling. Anal. Bioresour. 10(2): 2376-2391 (16 pages).

Fazal, S.; Zhang, B.P.; Zhong, Z.X.; Gao, L.; Chen, X.C., (2015). Industrial wastewater treatment by using membrane ioreactor: Review study. J. Environ. Protect., 6: 584-598 (15 pages).

Ingole, N.W.; Bhole, A.G., (2003). Removal of heavy metals from aqueous solution by water hyacinth: Azolla caroliniana. J. Water SRT– Aqua, 52: 119-128 (10 pages).

Ishtiyak, Q.; Chhipa, R.C., (2015). Critical evaluation of some available treatment techniques for textile and paper industry effluents: A Rev. Am. Chem. Sci. J., 6(2): 77-90 (14 pages).

Jarpa, M.; Rozas, O.; Salazar, C.; Baeza, C.; Campos, J.L.; Mansilla, H.D.; Vidal, G., (2015). Comparison of the chemical precipitation, UV/H2O2 and fenton processes to optimize removal of chronic toxicity from kraft mill effluents. Desalination Water Treat., 10.1080/19443994.2015.1061454

Kesalkar, V.P.; Isha, P.; Sudame, A.M., (2012). Physico-chemical characteristics of wastewater from Paper Industry, Int. J. Eng. Res. Appl., 2(4): 137-143 (8 pages).

Salmah, B.; Karman, S.; Zaleha M. D. ; Ille, C. G.,  (2015). Raw Materials Synthesis from Heavy Metal Industry Effluents with Bioremediation and Phytomining : A Biomimetic Resource Management Approach. Adv. Mater. Sci. Eng. Article ID 185071, 21 pp.,

Shankar, D.; Sivakumar, M.; Thiruvengadam, M.; Manojkumar, (2014b). Color removal in a textile industry wastewater using coconut coir pith. Pollut. Res. 33: 490–503 (14 pages).

Shankar, D.; Sivakumar, D.; Yuvashree, R., (2014j). Chromium (VI) removal from tannery industry wastewater using fungi species. Pollut. Res., 33: 505–510 (6 pages).

Sivakumar, D., (2011). A study on contaminant migration of sugarcane effluent through porous soil medium, Int. J. Environ. Sci. Tech., 8: 593–604 (10 pages).

Sivakumar, D.,(2014d).Role of low cost agro-based adsorbent to treat hospital wastewater, Pollut. Res., 33: 573–576 (4 pages).

Sivakumar, D.; Kandaswamy, A.N.; Gomathi, V.; Rajeshwaran,R.; Murugan, N., (2014i). Bioremediation studies on reduction of heavy metals toxicity, Pollut. Res. 33: 553–558 (6 pages).

Sivakumar, D.; Shankar, D.;  Vijaya Prathima, A.J.R.; Valarmathi, M., (2013c). Constructed wetlands treatment of textile industry wastewater using aquatic macrophytes. Int. J. Environ. Sci., 3: 1223–1232 (10 pages).

Sivakumar, D.;  Shankar, D.;  Dhivya, P.; Balasubramanian, K., (2014h). Bioaccumulation study by lemna gibba lin., Pollut. Res., 33: 531–536 (6 pages).

Sivakumar, D.; Shankar, D.; Nithya, S.; Rajaganapathy, J., (2014e). Reduction of contaminants from leachate using moringa oleifera: A kinetic study. Pollut. Res. 33: 529-529 (1 pages).

Sivakumar, D.; Shankar, D.; Gomathi, V.; Nandakumaar, A., (2014g). Application of electro-dialysis on removal of heavy metals, Pollut. Res. 33: 627–637 (11 pages).

Sivakumar, D.; Gayathri, G.; Nishanthi, R.;  Vijayabharathi, V.; Sudeshna, D.;  Kavitha, R., (2014k). Role of fungi species in colour removal from textile industry wastewater, Int. J. Chem. Tech. Res. 6: 4366–4372 (7 pages).

Sivakumar, D.;  Murugan, N.; Rajeshwaran, R.; Shobana, T.; Soundarya, C.; Vanitha, V.S., (2014a). Role of rice husk silica powder for removing Cr(VI) in a tannery industry wastewater, Int. J. Chem.Tech. Res. 6: 4373–4378 (6 pages).

Sivakumar, D.;  Shankar, D., (2012).  Color removal from textile industry wastewater using low cost adsorbents, Int. J. Chem. Environ. Pharmaceutical Res., 3: 52–57 (6 pages).

Sivakumar, D.; Balasundaram, V.; Venkatesan, G.; Saravanan, S.P., (2014c). Effect of tamarind kernelpowder for treating dairy industry wastewater. Pollut. Res., 33: 519–523 (5 pages).

Soltan, M.E.; Rashed, M.N., (2003).  Laboratory study on the survival of water hyacinth under several conditions of heavy metal concentrations. Adv. Environ. Res., 7: 82- 91 (10 pages).

Türker, O.C.; Vymazal, J.V.; Türe, C., (2014). Constructed wetlands for boron removal: A review. Ecol. Eng., 64: 350-359 (10 pages).

Sivakumar, D., (2013a). Experimental and analytical model studies on leachate volume computation from solid waste, Int. J. Environ. Sci. Tech., 10: 903–916.

Sivakumar Durairaj, (2013b), Adsorption study on municipal solid waste leachate using moringa oleifera seed, Int. J. Environ. Sci. Technol., Vol. 10, pp. 113–124 (12 pages).

Sivakumar, D.; Shankar, D.; Kandaswamy, A.N.; Ammaiappan, M., (2014f). Role of electro-dialysis and electro-dialysis cum adsorption for chromium (VI) reduction, Pollut. Res., 33: 547–552 (7 pages).

Swamy, N.K.; Singh. P.; Sarethy, I.P., (2011). Precipitation of phenols from paper industry wastewater using ferric chloride. Rasayan J. Chem., 4(2): 452-456 (6 pages).

Vinod Kumar, A.K.; Chopra, S.K.; Jogendra, S. Roushan, K. T. (2015). Effects of pulp and paper mill effluent disposal on soil characteristics in the vicinity of Uttaranchal Pulp and Paper Mill, Haridwar (Uttarakhand), India, Int. J. Agric. Sci. Res. 4(6): 117-125 (9 pages).

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