Microbial Sciences Division, Agharkar Research Institute, G.G. Agharkar Road, Pune 411004, India


Glycerol is a promising feedstock for microbial cultivation and production of 1,3 propanediol (1,3 PDO). Here we report a newly isolated bacterial strain BA11 from soil, capable of fermenting glycerol to 1,3 PDO, and has been identified to be a strain of Klebsiella pneumoniae. Strain BA11 was fast growing showing peak 1,3 PDO production in 6 h of cultivation with productivity of 1.2 g/L-h without the addition of Vitamin B12. Based on the optimum glycerol utilization (75%) and 1,3 PDO production (8.3 g/L) and yield (0.56 mol/mol glycerol utilized), the most appropriate glycerol concentration for cultivation was 20 g/L. The strain BA11 could tolerate the pH range of 6 to 8.5 as no inhibitory effects were seen on growth as well as 1,3 PDO production. Strain BA11 was most active and could produce high 1,3 PDO in the incubation temperature range of 25 to 40 oC. The production of 1,3 PDO was maximum (9.3 g/L) under aerobic condition with 95.8% glycerol utilization. Addition of glucose to the glycerol fermentation led to increased cell mass but no improvement in the 1,3 PDO production.


Abbad-Andaloussi, S.; Amine, J.; Gerard, P.; Petitdemange, H., (1998). Effect of glucose on glycerol metabolism by Clostridium butyricum DSM 5431, J. Appl. Microbiol., (84): 515-522 (8 pages).

Asad Ur, R.; Saman, W.R.G.; Nomura, N.; Sato, S.; Matsumura, M., (2008). Pre-treatment and utilization of raw glycerol from sunflower oil biodiesel for growth and 1,3-propanediol production by Clostridium butyricum, J. Chem. Tech. Biotech., (83): 1072-1080 (9 pages).

Baeza-Jimenez, R.; Lopez-Martinez, L .X.; De La Cruz-Medina, J.; Espinosa-De-Los-Monteros, J.J.; Garcia-Galindo, H.S., (2011). Effect of glucose on 1,3-propanediol production by Lactobacillus reuteri, Revista Mexicana De Ingenieria Quimica, (10): 39-46 (8 pages).

Biebl, H., (2001). Fermentation of glycerol by Clostridium pasteurianum—batch and continuous culture studies, J. Ind. Microbiol. Biotechnol., (27): 18-26 (9 pages).

Biebl, H.; Marten, S., (1995). Fermentation of glycerol to 1,3-propanediol : use of cosubstrates, Appl. Microbiol. Biotechnol., (44): 15-19 (5 pages).

Brenner, D.J.; Krieg, N.R.; Staley, J.T., (2005). Bergey’s Manual of Systematic Bacteriology, Williams & Wilkins, 1984. Chatzifragkou, A.; Papanikolaou, S.; Dietz, D.; Doulgeraki, A.I.; Nychas, G.J.E; Zeng, A.P., (2011). Production of 1,3-propanediol by Clostridium butyricum growing on biodiesel-derived crude glycerol through a non-sterilized fermentation process, Appl. Microbiol. Biotechnol., (91): 101-112 (12 pages).

Chen, X.; Xiu, Z.; Wang, J.; Zhang, D.; Xu, P., (2003). Stoichiometric analysis and experimental investigation of glycerol bioconversion to 1,3-propanediol by Klebsiella pneumoniae under microaerobic conditions, Enzyme Microb. Technol., (33): 386-394 (9 pages).

Cheng, K.K.; Zhang, J.A.; Liu, D.H.; Sun, Y.; Yang, M.D.; Xu, J.M., (2006). Production of 1,3-propanediol by Klebsiella pneumoniae from glycerol broth, Biotechnol, Lett., (28): 1817-21 (5 pages).

Clomburg, J.M.; Gonzalez, R., (2013). Anaerobic fermentation of glycerol: A platform for renewable fuels and chemicals, Trends Biotechnol., (31): 20-28 (9 pages).

Da Silva, G.P.; Mack, M.; Contiero, J., (2009). Glycerol: a promising and abundant carbon source for industrial microbiology, Biotechnol. advances, (27): 30-39 (10 pages).

Dobson, R.; Gray, V.; Rumbold, K., (2012). Microbial utilization of crude glycerol for the production of value-added products, J. Ind. Microbiol. Biotechnol., (39): 217-226 (10 pages).

Forsberg, C.W., (1987). Production of 1, 3-Propanediol from Glycerol by Clostridium acetobutylicum and Other Clostridium Species, Appl. Environ. Microbiol., (53): 639-643 (5 pages).

González-Pajuelo, M.; Andrade, J.C.; Vasconcelos, I., (2004). Production of 1,3-propanediol by Clostridium butyricum VPI 3266 using a synthetic medium and raw glycerol, J. Ind. Microbiol. Biotechnol., (31): 442-446 (5 pages).

González-Pajuelo, M.; Andrade, J.C.; Vasconcelos, I., (2005). VPI 3266 in continuous cultures with high yield and productivity, J. Ind. Microbiol. Biotechnol., (32): 391-396 (6 pages).

Holdeman, L.V.; Moore, W.E.C., (1975). Anaerobic Laboratory Manual Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA. Khanna, S.; Goyal, A.; Moholkar, V. S., (2012). Microbial conversion of glycerol: Present status and future prospects,

Critical Reviews in Biotechnology, (32): 235-262 (28 pages).

Mu, Y.; Teng, H.; Zhang, D.J.; Wang, W.; Xiu, Z.L., (2006). Microbial production of 1,3-propanediol by Klebsiella pneumoniae using crude glycerol from biodiesel preparations, Biotechnol. Lett., (28): 1755-1759 (5 pages).

Papanikolaou, S.; Aggelis, G., (2003). Modelling aspects of the biotechnological valorization of raw glycerol: production of citric acid by Yarrowia lipolytica and 1,3-propanediol by Clostridium butyricum, J. Chem. Technol. Biotechnol., (78): 542-547 (6 pages).

Papanikolaou, S.; Fakas, S.; Fick, M.; Chevalot, I.; Galiotou- Panayotou, M.; Komaitis, M.; Marc, I.; Aggelis, G., (2008). Biotechnological valorisation of raw glycerol discharged after bio-diesel (fatty acid methyl esters) manufacturing process: Production of 1,3-propanediol, citric acid and single cell oil, Biomass Bioenergy, (32): 60-71 (12 pages).

Papanikolaou, S.; Fick, M.; Aggelis, G., (2004). The effect of raw glycerol concentration on the production of 1,3-propanediol by Clostridium butyricum, J. Chem. Technol. Biotechnol., (79): 1189-1196 (8 pages).

Sambrook, J.; Russell, D.W., (2001). Molecular cloning – A laboratory manual Cold Spring Harbor Laboratory Press, Cold Spring Harbor. Saxena, R.K.; Anand, P.; Saran, S.; Isar, J., (2009). Microbial production of 1,3-propanediol: Recent developments and emerging opportunities, Biotechnol. advances, (27): 895-913 (19 pages).

Tamura, K.; Dudley, J.; Nei, M.; Kumar, S., (2007). MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0, Mol. Bio. Evol., (24): 1596-1599 (4 pages).

Temudo, M.F.; Poldermans, R.; Kleerebezem, R.; Van Loosdrecht, M.C.M., (2008). Glycerol fermentation by (open) mixed cultures: a chemostat study, Biotechnol. Bioeng., (100): 1088-98 (11 pages).

Xiu, Z.; Chen, X.; Sun, Y.; Zhang, D., (2007). Stoichiometric analysis and experimental investigation of glycerol–glucose co-fermentation in Klebsiella pneumoniae under microaerobic conditions, Biochem. Eng. J., (33): 42-52 (11 pages).

Xu, Y.Z.; Wu, R.C.; Zheng, Z.M.; Liu, D.H., (2011). Influence of dhaT mutation of K. pneumoniae on 1,3-propanediol fermentation, World J. Microbiol. Biotech., (27): 1491-1497 (7 pages).

Zhang, G.; Ma, B.; Xu, X.; Li, C.; Wang, L., (2007). Fast conversion of glycerol to 1 , 3-propanediol by a new strain of Klebsiella pneumoniae, Biochem. Eng. J., (37): 256-260 (5 pages).

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