1 Department of Chemical Engineering, Faculty of Energy, Kermanshah University of Technology, Kermanshah, Iran

2 Department of Environmental Sciences, Faculty of Natural Resources, University of Zabol, Zabol, Iran


phenol and phenolic compounds are among the most recognized environmental pollutants which exist in industrial wastewater and enter the biological cycles due to the solubility in water. Bioremediation is one of the cost-effective and Eco-friendly methods for phenol removal. In this study, the most effective phenol-degrading bacterial strain was isolated and identified from the shores of the Oman Sea by 16S rDNA. The optimal conditions of various factors, such as pH, temperature, carbon to nitrogen ratio and salinity for the phenol biodegradation, were determined using the experimental design based on Taguchi method with L9 array (34). Ability of the isolated strain (Halomonas elongata strain O-CH1) in degradation of different phenol concentrations was analyzed. The optimum operating conditions for phenol removal were determined in pH value of 8, temperature of 35 ˚C, carbon to nitrogen ratio of 100:30 (g/L) and salinity of 35 (g/L). In these conditions, 97% of the phenol was removed from the mediums. According to the optimization results, salinity and pH were the most influential factors in the biodegradation of phenol. The O-CH1 was able to grow and degrade phenol at concentrations of 250 mg/L to 1500 mg/L. Considering the high potential of this strain for phenol degradation, determining the optimal conditions for the biodegradation and its efficacy at high concentrations of phenol, the findings in this study can be used in the biological treatment of phenolic wastewater.

Graphical Abstract


  • The Halomonas elongata O-CH1 as the most effective phenol-degrading bacterial strain was isolated and purified from the Oman Sea-Chabahar coast;
  • The optimum operating conditions for the phenol removal by strain O-CH1 were successfully determined using the Taguchi method;
  • Strain O-CH1 can be used in bioremediation of the water contaminated with phenolic compounds.


Main Subjects

APHA., (1998). Standard methods for the examination of water and wastewater. Joint publication of the American Public Health Association, the American Water Works Association and the Water Environment Federation.

Atlas, R.M., (1981). Microbial degradation of petroleum hydrocarbons. An environmental perspective. Microbiol Rev., 45(1): 180-209 (30 pages).

Bao, M.T.; Wang, L.N.; Sun, P.Y.; Cao, L.X.; Zou, J.; Li, Y.M., (2012). Biodegradation of crude oil using an efficient microbial consortium in a simulated marine environment. Mar. Pollut. Bull., 64(6): 1177-1185 (9 pages).

Basak, B., Bhunia, B., Dutta, S. and Dey, A., (2013). Enhanced biodegradation of 4-chlorophenol by Candida tropicalis PHB5 via optimization of physicochemical parameters using Taguchi orthogonal array approach. Int . Biodeterior Biodegrad., 78: 17-23 (7 pages).

Basak, B.; Bhunia, B.; Mukherjee, S.; Dey, A., (2013). Optimization of physicochemical parameters for phenol biodegradation by Candida tropicalis PHB5 using Taguchi methodology. Desalin. Water Treat. J., 51(34-36): 6846-6862 (17 pages).

Bonfá, M.R.L.; Grossman, M.J.; Piubeli, F.; Mellado, E.;  Durrant, L.R., (2013). Phenol degradation by halophilic bacteria isolated from hypersaline environments. Biodegradation., 24(5): 699-709 (11 pages).

Calvo, C.; Martinez-checa, F.; Toledo, F.L.; Porcel, J.; Quesada, E., (2002). Characteristics of bioemulsifiers synthesised in crude oil media by Halomonas eurihalina and their effevtiveness in the isolation of bacteria able to grow in the presence of hydrocarbons. Appl. Microbiol. Biotechnol., 60(3): 347-351 (5 pages).

Casellas, M.; Grifoll, M.; Sebate, J.; Solanas, A. M.; (1998). Isolation and characterization of a 9-fluorenone-degrading bacterial strain and its role in synergistic degradation of fluorene by a consortium. Can. J. Microbiol., 44(8): 734-742 (9 pages).

Chen, J.; Wong, M.H.; Wong, Y.S.; Tam, N.F.Y., (2008). Multi-factors on biodegradation kinetics of polycyclic aromatic hydrocarbons (PAHs) by Sphingomonas sp., a bacterial strain isolated from mangrove sediment. Mar. Pollut. Bull., 57(6-12): 695-702 (8 pages).

Daneshvar, N.; Khataee, A.R.; Rasoulifard, M.H.; Pourhassan, M., (2007). Biodegradation of dye solution containing Malachite Green: Optimization of effective parameters using Taguchi method. J. Hazard. Mater., 143(1-2): 214-219 (6 pages).

Deng, T.; Wang, H.; Yang, K., (2018). Phenol biodegradation by isolated Citrobacter strain under hypersaline conditions. Water Sci. Technol., 77(2): 504-510 (7 pages).

Haddadi, A.; Shavandi, M., (2013). Biodegradation of phenol in hypersaline conditions by Halomonas sp. strain PH2-2 isolated from saline soil. Int. Biodeterior Biodegrad., 85: 29-34 (6 pages).

Hasan, S.A.; Jabeen, S., (2015). Degradation kinetics and pathway of phenol by Pseudomonas and Bacillus species. Biotechnol Biotechnol Equip., 29(1): 45-53 (9 pages).

Hossen, M.Z.; Hussain, M.E.; Hakim, A.; Islam, K.; Uddin, M.N;  Azad, A.K., 2019. Biodegradation of reactive textile dye Novacron Super Black G by free cells of newly isolated Alcaligenes faecalis AZ26 and Bacillus spp obtained from textile effluents. Heliyon., 5(7), e02068 (30 pages).

Hsien T.Y.; Lin, Y.H., (2005). Biodegradation of phenolic wastewater in a fixed biofilm reactor, Biochem. Eng. J., 27(2): 95-103 (9 pages).

Jiang, Y.; Yang, K.; Wang, H.; Shang, Y.; Yang, X., (2015). Characteristics of phenol degradation in saline conditions of a halophilic strain JS3 isolated from industrial activated sludge. Mar. Pollut. Bull., 99(1-2): 230-234 (5 pages).

Kim, Y.H.; Freeman, J.P., (2005). Effects of pH on the degradation of phenanthrene and pyrene by Mycobacterium vanbaalenii PYR-1. Appl. Microbiol. Biotechnol., 67(2): 275-285 (11 pages).

Kumar, A.; Bhunia, B.; Dasgupta, D.; Mandal, T.; Dey, A.; Datta, S.; Bhattacharya, P., (2013). Optimization of culture condition for growth and phenol degradation by Alcaligenes faecalis JF339228 using Taguchi Methodology. Desalin Water Treat., 51(16-18): 3153-3163 (11 pages).

Kumar, A.; Kumar, S.; Kumar, S., (2005). Biodegradation kinetics of phenol and catechol using Pseudomonas putida MTCC 1194. Biochem. Eng. J., 22(2): 151-159 (9 pages).

Leahy, J.G.; Colwell, R.R., (1990). Microbial degradation of hydrocarbons in the environment. Microbiol. Rev., 54(3): 305-315 (11 pages).

Leys, N.M.; Bastiaens, L.; Verstraete, W.; Springael, D., (2005). Influence of the carbon/nitrogen/phosphorus ratio on polycyclic aromatic hydrocarbon degradation by Mycobacterium and Sphingomonas in soil. Appl. Microbiol. Biotechnol., 66(6): 726-736 (11 pages).

Lin, C.; Gan, L.; Chen, Z. L., (2010). Biodegradation of naphthalene by strain Bacillus fusiformis (BFN). J. Hazard. Mater., 182(1-3): 771-777 (7 pages).

Margesin, R; Schinner, F., (2001). Biodegradation and bioremediation of hydrocarbons in extreme environments. Appl . Microbiol. Biotechnol., 56(5-6): 650-663 (14 pages).

Mnif, S.; Chamkha, M.; Sayadi, S., (2009). Isolation and characterization of Halomonas sp. Strain C2SS100, a hydrocarbon–degrading bacterium under hypersaline conditions. J. Appl. Microbiol., 107(3): 785-794 (10 pages).

Mohajeri, L.; Aziz, H.A.; Isa, M.H.; Zahed, M.A., (2010). A statistical experiment design approach for optimizing biodegradation of weathered crude oil in coastal sediments. Bioresour. Technol., 101 (3): 893-900 (8 pages).

Montgomery, D.C., (2017). Design and analysis of experiments, John wiley and sons, (630 pages).

Okpokwasili, G.C.; Nweke, C.O., (2006). Microbial growth and substrate utilization kinetics. Afr. J. Biotechnol., 5(4): 305-317 (13 pages).

Pant, A.; Rai, J.P.N., (2018). Bioremediation of chlorpyrifos contaminated soil by two phase bioslurry reactor: Processes evaluation and optimization by Taguchi's design of experimental (DOE) methodology. Ecotoxicol. Environ. Saf., 150: 305-311 (7 pages).

Peytone, B.M.; Oie, C.S.; Albaugh, C.E., (2007). Benzoate and salicylate degradation by Halomonas campisalis, an alkaliphilic and moderately halophilic microorganism. Water Res., 41(6): 1235-1242 (8 pages).

Ren, L.F.; Chen, R.; Zhang, X.; Shao, J.; He, Y., (2017). Phenol biodegradation and microbial community dynamics in extractive membrane bioreactor (EMBR) for phenol-laden saline wastewater. Bioresour. Technol., 244(1): 1121-1128 (8 pages).

Rucká, L.; Nešvera, J.; Pátek, M., (2017). Biodegradation of phenol and its derivatives by engineered bacteria: current knowledge and perspectives. World J. Microbiol. Biotechnol., 33(9): 174 (8 pages).

Sadeghi Haddad Zavareh, M.; Ebrahimipour, G.; Sahahriari Moghadam, M.; Fakhari,J.; Abdoli, T., (2016). Bioremediation of Crude Oil Using Bacterium from the Coastal Sediments of Kish Island, Iran. Iran. J. Public Health. 45(5): 670-679 (10 pages).

Samimi, M.; Shahriari Moghadam, M., (2018). Optimal conditions for biological removal of ammonia from wastewater of a petrochemical plant using the response surface methodology. Global J. Environ. Sci. Manage., 4(3): 315-324 (10 pages).

Sugiura, K.; Ishihara, M.; Shimauchi, T.; Harayama, S., (1996). Physicochemical properties and biodegradability of crude oil. Environ. Sci. Technol., 31(1): 45-51 (7 pages).

Schlegel, HG., (1992). Allgemeine Mikrobiologie: Auflage, Georg Thieme Verlag (655 pages).

Shahriari Moghadam, M.; Ebrahimipour, G.; Abtahi, B.; Ghassempour, A.; Hashtroudi, M.S., (2014). Biodegradation of polycyclic aromatic hydrocarbons by a bacterial consortium enriched from mangrove sediments. J. Environ. Health Sci. Eng., 12(1): 1-9 (9 pages).

Shahriari Moghadam, M.; Ebrahimipour, G.; Abtahi, B.; Khazaei, N.; Karbasi, N., (2014). Statistical optimization of crude oil biodegradation by Marinobacter sp. isolated from Qeshm Island, Iran. Iran J. Biotechnol., 12(1):35-41 (7 pages).

Shahriari Moghadam, M.; Safaei, N.; Ebrahimipour, G.H., (2016). Optimization of phenol biodegradation by efficient bacteria isolated from petrochemical effluents. Global J. Environ. Sci. Manage., 2(3): 249-256 (8 pages).

Sepehr, S.; Shahnavaz, B.; Asoodeh, A.; Karrabi, M., 2019. Biodegradation of phenol by cold-tolerant bacteria isolated from alpine soils of Binaloud Mountains in Iran. J. Environ. Sci. Health. Part A., 54(4): 367-379 (13 pages).

Tena-Garitaonaindia, M.; Llamas, I.; Toral, L.; Sampedro, I., 2019. Chemotaxis of halophilic bacterium Halomonas anticariensis FP35 towards the environmental pollutants phenol and naphthalene. Sci Total Environ., 669: 631-636 (6 pages).

Tremblay, L.; Kohl, S.D.; Rice, J.A.; Gagne, J.P., (2005). Effects of temperature, salinity, and dissolved humic substances on the sorption of polycyclic aromatic hydrocarbons to estuarine particles. Mar. Chem., 96(1-2): 21-34 (14 pages).

Yin, Y.; Wang, Y.; Tang, W.; Song, L., (2017). Thauera phenolivorans sp. nov., a phenol degrading bacterium isolated from activated sludge. Antonie van Leeuwenhoek., 110(12):1681-1690 (10 pages).

Yuan, S.Y.; Chang, J.S.; Yue, J.H.; Chang, B.V., (2001). Biodegradation of phenanthrene in river sediment. Chemosphere., 43(3): 273-278 (6 pages).

Youssef, M.; El-Shatoury, E.H.; Ali, S.S.; El-Taweel, G.E., 2019. Enhancement of phenol degradation by free and immobilized mixed culture of Providencia stuartii PL4 and Pseudomonas aeruginosa PDM isolated from activated sludge. Bioremediat J., 1-19 (19 pages).



Samimi, M.; Shahriari Moghadam, M., (2020). Phenol biodegradation by bacterial strain O-CH1 isolated from seashore. Global J. Environ. Sci. Manage., 6(1): …,

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