Different methods of bacterial inoculation on the yield of chamomile blossoms and essential oil

Mostafa, A.; Khalafallah, M.; AboSedera, S.; Fathy, H.; Higazy, A.

doi:10.22034/gjesm.2019.02.09

Document Type : ORIGINAL RESEARCH ARTICLE

Authors

¹ Department of Agricultural Microbiology, Agricultural and Biological Research Division, National Research Center, Egypt

² Department of Agricultural Microbiology, Faculty of Agriculture, Cairo University, Egypt

https://doi.org/10.22034/gjesm.2019.02.09

Abstract

Chamomile is one of the most wide spread medicinal plant cultivated in Egypt. This work aimed at enhancement of blossoms and oil production of chamomile plants via biofertilization with PGPRs under organic farming system. In this study, 6 bacterial strains were applied using two different inoculation techniques. The first application method was throughout soaking the roots of seedlings in the bacterial suspension before transplanting. The second technique was by adding the bacterial inocula to soil 2 weeks after transplantation. The results showed that root dipping method displayed high impact on the yield of chamomile blossoms and essential oil percentage. Furthermore, the soil application of the bacterial inocula didn’t show any significant impact in this respect. Where Paenibacillus polymyxa, Bacillus subtilis, Serratia plymuthica and Streptomyces subrutilus increased the dry weight of chamomile blossoms compared to the control, essential oil content increased significantly in case of Serratia plymuthica, Stenotrophomonas rhizophyla and Bacillus subtilis. The current results also indicated that bacterial strains produced the highest indole-3-acetic acid and gibberellic acid resulted in the highest yield of both flowers and essential oil.

Graphical Abstract

Highlights

Improvement of chamomile plant production through biofertilization under organic farming system was evaluated;
Chamomile blossom and oil yield enhanced by submerging the seedlings root in the Plant growth promoting rhizobacteria suspension;
Bacterial strains producing phytohormones showed the positive impact on blossoms and oil yield.

Keywords

Main Subjects

Environmental microbiology

References

Alvarenga, M.A.R., (2004). Produçãoemhidroponia. In: ALVARENGA, MAR (Ed.). Tomate: produçãoem campo, em casa de vegetaçãoeemhidroponia. Lavras: UFLA, 1(1): 191-212 (22 pages).

Amal, Z. El-A.M., (2015). Economic study on the production and exports of Egyptian chamomile. Int. J. Basic Appl. Sci., 5(2): 27-39 (13 pages).

Amiri, S.; Sharafzadeh, S.; Ordookhani, K., (2014). The Effect of Gibberellic Acid and Benzyladenine on Growth and Essential Oils of German Chamomile. Ind. J. Fundament. Appl. Life Sci., 4(1):186-188 (3 pages).

Bano, U.; Khan, A.F.; Mujeeb, F.; Maurya, N.; Tabassum, H.; Siddiqui, M.H.; Haneef, M.; Osama, K.; Farooqui, A., (2016). Effect of plant growth regulators on essential oil yield in aromatic plants. J. Chem. Pharm. Res., 8(7):733-739 (7 pages).

Bashan, Y.; Holguin, C., (1997). Azospirillum-plant relationships: Environmental and physiological advances (1990–1996). Can. J. Microbiol., 43 (2): 103–121 (19 pages).

Eltayeb, F.M.E., (2017). Biological Control of root knot disease of tomato caused by Meloidogyne javanica using Pseudomonas fluorescens bacteria. Int. J. Curr. Microbiol. App. Sci., 6(6): 1176-1182 (7 pages).

Farkoosh, S.S.; Ardakani, M.Z.; Rejali,F.; Darzi, M.T.; Faregh, A.H., (2011). Effect of Mycorrhizal Symbiosis and Bacillus coagolance on Qualitative and Quantitative Traits of Matricaria chamomilla under Different Levels of Phosphorus. Middle-East J. Sci. Res. 8 (1): 01-09 (9 pages).

Gosztola, B., (2012). Morphological and chemical diversity of different chamomile (Matricaria recutita L.) populations of the great Hungarian plain. Ph.D. Thesis, Fac. Horticult. Sci., Corvinus University of Budabest (16 pages).

Grosch, R.; Faltin, F.; Lottmann, J.; Kofoet, A.; Berg, G., (2005). Effectiveness of 3 antagonistic bacterial isolates to control Rhizoctonia solani Kühnonlettuce and potato. Can. J.Microbiol. 51: 345–353 (9 pages).

Gutierrez-Manero F.J.; Ramos, B.; Probanza, A.; Mehouachi, J.; Talon, M., (2001). The plant growth promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberelins. Physiol. Plant, 111: 206–211 (6 pages).

Hamed, E.S.; Toaima, W.I.M.; El-Shazly, M., (2017). Effect of planting density and biofertilization on growth and productivity of Cymbopogon citratus (DC.) Stapf. (Lemongrass) plant under Siwa Oasis conditions. J. Med. Plants Stud., 5(2): 195-203 (9 pages).

Hazzoumi, Z.; Moustakime, Y.; Joutei, K.A., (2014). Effect of gibberellic acid (GA), indole acetic acid (IAA) and benzylaminopurine (BAP) on the synthesis of essential oils and the isomerization of methyl chavicol and trans-anethole in Ocimum gratissimum L. Springer Plus, 3: 321 (7 pages).

Idris, E.E.S.; Iglesias, D.J.; Talon, M.; Borriss, R., (2007). Tryptophan-dependent production of Indole-3- Acetic Acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42. Mol. Plant Microbe Interact., 20: 619–626 (8 pages).

Kamensky, M.; Ovadis, M.; Chet, I.; Chernin, L., (2003). Soil-borne strain IC14 of Serratia plymuthica with multiple mechanisms of antifungal activity provides biocontrol of Botrytis cinerea and Sclerotinia sclerotiorum diseases. Soil Biol. Biochem., 35: 323–331 (9 pages).

Khalil, N.H.; Agah, R.J., (2017). Effect of chemical, organic and bio-fertilization on growth and yield of strawberry plant. Int. J. Adv. Chem. Eng. Biol. Sci., 4(1) (5 pages).

Khan, A. F.;Mujeeb, F.; Aha, F.; Farooqui, A., (2015). Effect of plant growth regulators on growth and Essential Oil Content in Palmarosa (Cymbopogon martinii). Asian J. Pharm. Clin. Res., 8(2): 373-376 (4 pages).

Köberl, M.; Muller, H.; Ramadan, E.M.; Berg, G., (2011). Desert farming benefits from microbial potential in arid soils and promotes diversity and plant health. PLoS ONE, 6(9): e24452 (9 pages).

Kumar, V.; Kumar, V.; Umrao, V.; Singh, M., (2012). Effect of Ga3 and IAA on growth and flowering of carnation. Hort. Flora Res. Spectrum, 1(1): 69-72 (4 pages).

Lottmann, J.; Berg, G., (2001). Phenotypic and genotypic characterization of antagonistic bacteria associated with roots of transgenic and non-transgenic potato plants. Microbiol. Res., 156: 75-82 (8 pages).

Mapeli, N.C.; Vieira, M.C.; Heredia Z.N.A.; Siqueira, J.M., (2005). Produção de biomassa e de óleo essencial dos capítulos florais da camomila em função de nitrogênio e fósforo. Horticultura Brasileira, Brasília, 23(1): 32-37 (6 pages).

Mehnaz, S.; Kowalik , T.; Reynolds, B.; Lazarovits, G. (2010). Growth promoting effects of corn (Zea mays) bacterial isolates under greenhouse and field conditions. Soil Biol. Biochem., 42: 1848-1856 (9 pages).

Mohammad, S.M., (2011). Study on Chammomile (Matricaria chamomilla L.) usage and Farming. Adv. Env. Biol., Iran, 5(7): 1446-1453 (8 pages).

Muller, A.; Duchting, P.; Weiler, E.W., (2002). A multiplex GC-MS/MS technique for the sensitive and quantitative single-run analysis of acidic phytohormones and related compounds, and its application to Arabidopsis thaliana. Planta, 216: 44–56 (13 pages).

Newall, C.A.; Anderson, L.A.; Phillipson, J.D., (1996). Herbal medicines: A guide for health care professionals. London: Pharm. Press, ix, 296 (296 pages).

Reda, F.; Abd El-Wahed M.S.A.; Gamal El Din K.M., (2010). Effect of indole acetic acid, Gibberellic acid and Kinetin on Vegetative Growth, Flowering, Essential Oil Pattern of Chamomile Plant (Chamomile recutita L. Rausch). World J. Agric. Sci., 6 (5): 595-600 (6 pages).

Richardson, A.E.;Barea J.M.; McNeill A.M.; Prigent-Combaret C., (2009). Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant Soil, 321:305–339 (35 pages).

Rodríguez, H.; Fraga, R., (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv., 17(1999): 319–339 (21 pages).

Salehi, A.; Gholamhoseini, M.; Ataei, R.; Sefikon, F.; Ghalavand, A., (2018). Effects of Zeolite, Bio- and Organic Fertilizers Application on German Chamomile Yield and Essential Oil Composition, J. Essent. Oil Bearing Plants, 21(1): 116-130 (15 pages).

Singh, A.; Bhat, S. S.;Pandey, Y.; Kumar, N. and Adarsh, A., (2015). Efficacy of auxins and gibberellic acid on floral attributes and essential oil per cent on German Chamomile (Matricaria chamomilla L.). Medicinal Plants, 7(4): 307-309 (3 pages).

Snedecor, G.W.; Cochran, W.G., (1967). Statistical Methods, Sixth ed. Iowa Univ. Press, Ames. Iowa, USA (593 pages).

Srivastava, J.K.; Shankar, E.; Gupta, S., (2010). Chamomile: A herbal medicine of the past with bright future. Mol. Med. Report., 3(6): 895–901 (13 pages).

Thomas, P.; Sekhar, A.C. (2016). Effects due to rhizospheric soil application of an antagonistic bacterial endophyte on native bacterial community and its survival in soil: A case study with Pseudomonas aeruginosa from Banana. Front. Microbiol. 7: 493 (16 pages).

Wei, G.; Kloepper, J. W.; Tuzun, S., (1991). Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth-promoting rhizobacteria. Phytopath., 81: 1508–12 (5 pages).

Whipps, J.M., (2001). Microbial interactions and biocontrol in the rhizosphere. J. Exp. Bot., 52: 487–511 (25 pages).

Wolf A.; Fritze A.; Hagemann M.; Berg G., (2002). Stenotrophomonas rhizophila sp. nov., a novel plant-associated bacterium with antifungal properties. Int. J. Syst. Evol. Microbiol., 52: 1937-1944 (8 pages).

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Global Journal of Environmental Science and Management

Different methods of bacterial inoculation on the yield of chamomile blossoms and essential oil

References

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Letters to Editor

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Volume 5, Issue 2 - Serial Number 18
April 2019
Pages 237-248

Different methods of bacterial inoculation on the yield of chamomile blossoms and essential oil

References

References

Letters to Editor

Send comment about this article

Volume 5, Issue 2 - Serial Number 18April 2019Pages 237-248

Volume 5, Issue 2 - Serial Number 18
April 2019
Pages 237-248