TY - JOUR ID - 9555 TI - Biochemical evaluation of antioxidant activity and polysaccharides fractions in seaweeds JO - Global Journal of Environmental Science and Management JA - GJESM LA - en SN - 2383-3572 AU - Tariq, A. AU - Athar, M. AU - Ara, J. AU - Sultana, V. AU - Ehteshamul-Haque, S. AU - Ahmad, M. AD - Department of Food Science and Technology, University of Karachi, Karachi-75270, Pakistan AD - California Department of Food and Agriculture, 3288 Meadowview Road, Sacramento, CA 95832, USA AD - Department of Biochemistry, University of Karachi, Karachi-75270, Pakistan AD - Department of Botany, University of Karachi, Karachi-75270, Pakistan AD - Department of Pharmacognosy, University of Karachi, Karachi-75270, Pakistan Y1 - 2015 PY - 2015 VL - 1 IS - 1 SP - 47 EP - 62 KW - Antioxidant Activity KW - Karachi coast KW - Polysaccharide fractions KW - Seaweeds DO - 10.7508/gjesm.2015.01.005 N2 - In the present study ethanol and water extracts of 15 seaweeds, Dictyota dichotoma var. velutricata, Dictyota indica, Iyengaria stellata, Padina pavonia, Sargassum swartzii, Sargassum variegatum, Stoechospermum marginatum, Stokeyia indica, Jolyna laminarioides, Caulerpa taxifolia, Halimeda tuna, Ulva fasciata, Ulva lactuca, Solieria robusta, and Melanothamnus afaqhusainii, were evaluated for their antioxidant potential by ABTS or 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), superoxide and total antioxidant capacity (TAC) assays.  The activity was concentration dependent and the variation in antioxidant potential was also observed by different assays in both extracts.  Ethanol extract of D. dichotoma var. velutricata, D. indica and S. marginatum demonstrated highest activity by TAC assay.  The antioxidant potential in organic solvent fractions of seaweeds namely P. pavonia, S. swartzii, S. marginatum and M. afaqhusainii was also determined and chloroform fraction of all the four seaweeds showed highest activity by superoxide assay.  Antioxidant activity of extracted fractions of polysaccharides from S. indica, C. taxifolia and D. dichotoma var. velutricata was also evaluated by superoxide method.  Polysaccharide fractions of S. indica obtained from HCl (at 700C and room temperature) and water extract demonstrated highest activity respectively.  All the polysaccharide fractions of C. taxifolia showed excellent activity except CaClF70°C. Polysaccharide fractions of D. dichotoma var. velutricata also exhibited very good activity. UR - https://www.gjesm.net/article_9555.html L1 - https://www.gjesm.net/article_9555_cb537ac8da1caad9c81324ca5401f531.pdf ER -