Environmental Science
E. Imelda; K. Khairan; R.R. Lubis; T. Karma; R. Idroes
Abstract
BACKGROUND AND OBJECTIVES: Isotoma longiflora L is commonly used as a medicinal plant by the local community in Indonesia, and its geographical position determines its bioactive compounds and hence its efficacy. Ethanol extracts of Isotoma longiflora leaves from various locations in Aceh Province were ...
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BACKGROUND AND OBJECTIVES: Isotoma longiflora L is commonly used as a medicinal plant by the local community in Indonesia, and its geographical position determines its bioactive compounds and hence its efficacy. Ethanol extracts of Isotoma longiflora leaves from various locations in Aceh Province were analyzed using a simple infrared spectroscopy technique combined with chemometrics to determine the effect of geographical location and conditions by classification and authentication.METHODS: Isotoma longiflora leaf samples were collected from Aceh Besar (a geothermal manifestation of Ie Suum), Banda Aceh, Aceh Jaya, Bireun, and Central Aceh. Principal component analysis was used to categorize the ethanol extract of Isotoma longiflora leaves, and a linear discriminant analysis was used for authentication.FINDINGS: The principal component analysis score plot indicated 89 percent of total data variance and that the samples formed three distinct groups: group I consisting of Aceh Tengah and Bener Meriah samples; group II of Aceh Besar and Banda Aceh samples; and group III of Aceh Selatan, Aceh Barat Daya, Aceh Jaya, and Bireun. A linear discriminant analysis was then used to validate these results, and the linear discriminant analysis model derived from the cross-validation predicted the origin of Isotoma longiflora samples with 100 percent accuracy rate.CONCLUSION: The Isotoma longiflora leaf extracts were successfully classified using Fourier-transform infrared spectroscopy data processed through chemometric calculations (namely, principal component analysis). Based on the cross-validation using linear discriminant analysis showed that the prediction model had a 100 percent accuracy. The present study thus revealed the effect of geographical location on the composition of bioactive compounds in Isotoma longiflora, suggesting the potential of chemometric techniques for quality control and assurance in traditional medicine.
Environmental Management
N.B. Maulydia; R. Idroes; K. Khairan; T.E. Tallei; F. Mohd Fauzi
Abstract
BACKGROUND AND OBJECTIVES: Tridax procumbens L. is a plant that grows abundantly in the Ie-Seu'um geothermal area in Aceh Province, Indonesia. The objective of this study is to determine metabolite compounds from Tridax procumbens plants in a geothermal area using qualitative and quantitative analyses. ...
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BACKGROUND AND OBJECTIVES: Tridax procumbens L. is a plant that grows abundantly in the Ie-Seu'um geothermal area in Aceh Province, Indonesia. The objective of this study is to determine metabolite compounds from Tridax procumbens plants in a geothermal area using qualitative and quantitative analyses. In addition, the contents of six heavy metals in plants and their toxicology were assessed using an in silico approach.METHODS: The ethanolic extract of Tridax procumbens was analyzed qualitatively using reagents to determine the contents of secondary metabolites such as flavonoids, alkaloids, tannins, steroids, triterpenoids, and saponins. In addition, quantitative analysis was conducted using gas chromatography–mass spectroscopy to obtain the chromatograms and mass spectra of the metabolite compounds of the ethanolic extract of Tridax procumbens, which were used in computational toxicology analysis using a simplified molecular input system in a predictor server. Atomic absorption spectrometry was conducted to confirm the contents of six heavy metals harmful to medicinal plants.FINDINGS: The results showed that Tridax procumbens from the Ie-Seu'um geothermal area, Aceh, has secondary metabolites such as flavonoids, saponins, steroids, and tannins, with phytol from diterpenoid group having the highest content (32.72 percent). Toxicological analysis showed that the compounds in the ethanolic extract of Tridax procumbens were nontoxic or inactive in five toxicity parameters. The other results of the heavy metal analysis showed the dominance of chromium among the other six metals tested (copper, not detected; cadmium, 0.91 ± 0.03 milligram per kilogram; zinc, 3.50 ± 0.03 milligram per kilogram; iron, 4.65 ± 0.02 milligram per kilogram; lead, 6.42 ± 0.05 milligram per kilogram; and chromium, 13.81 ± 0.07 milligram per kilogram.CONCLUSION: This study highlights the unique secondary metabolite composition of Tridax procumbens under such extreme conditions and underscores the potential implications of heavy metal accumulation in plants in geothermal areas.
Environmental Management
A. Abubakar; H. Yusuf; M. Syukri; R. Nasution; M. Yusuf; R. Idroes
Abstract
BACKGROUND AND OBJECTIVES: Medicinal plants growing in geothermal areas have been reported to possess relatively high concentrations of bioactive secondary metabolites, which have been attributed to the adaptive heat stress response. Nonetheless, the exploitation of their medicinal benefits is limited ...
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BACKGROUND AND OBJECTIVES: Medicinal plants growing in geothermal areas have been reported to possess relatively high concentrations of bioactive secondary metabolites, which have been attributed to the adaptive heat stress response. Nonetheless, the exploitation of their medicinal benefits is limited by potentially life-threatening concentrations of heavy metals. Chromolaena odorata Linn, also called seurapoh, is a well-known medicinal plant that can absorb and accumulate heavy metals from the soil. This present study aimed to investigate the contents of mercury, lead, cadmium, and arsenic in the ethanolic extract of Chromolaena odorata Linn leaves collected from a geothermal area in Aceh Province, Indonesia.METHODS: Three hot springs, namely Ie-Suum, Ie-Jue, and Ie-Brouk, located in the same geothermal area, Seulawah Agam, were selected as the sampling points, and three samples of Chromolaena odorata were collected at each point. Extraction was carried out by means of maceration employing ethanol solvent. The heavy metal contents of each extract were determined using priorly validated atomic absorption spectrometry and graphite furnace atomic absorption spectrometry. The maximum thresholds of each heavy metal from the Indonesian Agency for Drug and Food Control were adopted during data interpretation.FINDINGS: The results revealed the presence of trace levels of arsenic (0.0482 ± 0.004 – 0.0639 ± 0.007 milligram per kilogram) and lead (0.0219 ± 0.004 – 0.0672 ± 0.006 milligram per kilogram), which did not exceed Indonesian maximum safety thresholds (≤5 and ≤10 milligram per kilogram, respectively). Mercury in all samples was not observable (limit of detection = 0.018 milligram per liter). Cadmium was observed in almost all samples with a concentration range of 0.0219 ± 0.005–1.1472 ± 0.006 milligram per kilogram, which exceeded the maximum threshold (0.3 milligram per liter).CONCLUSION: Heavy metal contamination in the ethanolic extract of geothermal Chromolaena odorata leaves has been attributed to volcanic activities. Among the heavy metals of concern, cadmium was the only one with a concentration exceeding the safety limit. The presence of cadmium in the extract at a high concentration could cause its translocation to the human body, eventually leading to multiple organ damage. Therefore, the extract of Chromolaena odorata leaves collected from a geothermal area should be consumed with caution for possible cadmium intoxication.
