1Department of Environmental Science, Faculty of Natural Resources and Environment, University of Birjand, Birjand, Iran
2Research Center, National Inland Water Aquaculture Institute, Anzali Port, Iran
The aim of this study was to evaluate the ability of microalgae Spirulina platensis and Chlorella vulgaris to remove nitrate and phosphate in aqueous solutions. Spirulina platensis and Chlorella vulgar is microalgae was collected in 1000 ml of municipal water and KNO3, K2HPO4 was added as sources of nitrate and phosphate in three different concentrations (0.25, 0.35 and 0.45g/L). During the growth period, the concentration of nitrate and phosphate was recorded at 1, 4, 6 and 8 days. The highest nitrate removal on the 8 day for Chlorella vulgaris was 89.80% at the treatment of 0.25g/L and for Spirulina platensis was 81.49% at the treatment of 0.25g/L. The highest phosphate removal for Spirulina platensis was 81.49% at the treatment of 0.45g/L and for Chlorella vulgaris was 88% at the treatment of 0.45g/L. The statistical results showed that the amount of phosphate and nitrate removal during different time periods by Chlorella vulgaris depicted a significant difference at P<0.01, while Spirulina platensis demonstrated a significant difference at P<0.05.Thus, Spirulina platensis and Chlorella vulgaris can be effectively used to remove nitrate and phosphate from effluent and waste water treatments, although it demands more research in different climatic conditions.
Ability of microalgae to remove nitrate and phosphate in aqueous solutions
High pH in microalgae medium causes to reduce the removal of nitrate and phosphate
Microalgae remove the high concentrations of nitrogen and phosphorus
Abhijna, U.G., (2016). Monitoring and assessment of a eutrophicated coastal lake using multivariate approaches. Global J. Environ. Sci. Manage., 2 (3): 275-288 (14 pages).
Afshari, A.; Yahyavi, M.; Shybk, H.; Abdolallyan, A., (2011). Check availabilityofTetraselmis suecica microalgae in municipal secondary wastewater treatment. Aquat. Fish., 2 (8): 1-8 (8 papges).
Ahmadpour, N.; Sayadi, M.H.; Fallahi, M.; Rezaei, M. R., (2014). Removal of phosphate by microalgae from municipal wastewater effluents: Lab Experiment. Biotechnology Tarbiat Modares University, 6 (2):40-50 (In Persian) (11 pages).
APHA, (1998). Standard methods for examination of water and wastewater. American Public Health Association Publication, Washington DC.
Aravantinou, A.F.; Theodorakopoulos, M. A.; Manariotis, I.D., (2013). Selection of microalgae for wastewater treatment and potential lipids production. Biores. Tech.,147: 130–134 (5 pages).
Aslan, S.; Kapdan, I.K., (2006). Batch kinetics of nitrogen and phosphorus removal from synthetic wastewater by algae. Ecol. Eng., 28:64–70 (7 pages).
Caswell, M.; Zilberman, D., (2002). Algol – culture. University of California at Berkeley., 6:1-12 (13 pages).
Cheunbarn, S.; Peerapornpisal. Y., (2010). Cultivation of Spirulina platensis using anaerobically swine wastewater treatment effluent. Int. J. Agricul. Bio.,12: 586–590 (5 pages).
Chngany, Z.; Modares, A.; Afsharzadeh, S., (2012).Wastewater treatment using micro-algae Spirulina platensis culture. First National Conference on Phytoremediation. Kerman, Iran 16 February.
De Morais, M. G.; Costa, J.A., (2007). Biofixation of carbon dioxide by Spirulina sp. and Scenedesmus obliquus cultivated in a three-stage serial tubular photobioreactor. J. Biotech., 129:439–445 (7 pages).
Dickinson, K.E.; Whitney, C. G.; McGinn, P.J., (2013). Nutrient remediation rates in municipal wastewater and their effect on biochemical composition of the microalga Scenedesmus sp. AMDD. Algal Res.,2: 127–134 (8 pages).
Khan, M.; Yoshida, N., (2008). Effect of L-glutamic acid on the growth and ammonium removal from ammonium solution and natural wastewater by Chlorella vulgaris NTM06. Biores. Tech.,99: 575–582 (8 pages).
Koening, L. M.; Demacedo, J.S., (2004). Urban secondary sewage: an alternative medium for the culture of Tetraselmis chuii(Prasinophyceae) and Dunaliella viridis (Chlorophyceae). Braz. Arch. Biol. Tech., 3:451-459 (9 pages).
Kshirsagar, A.D., (2013). Bioremediation of wastewater by using microalgae, an experimental study. Int. J. Life Sci. Biotech. Pharma. Res., 2: 339–346 (7 pages).
Mousavi, A.; Yahyavi, M.; Taherizadeh, M., (2009). Evaluation of Chlorella vulgarisphytoplankton growth and its effects on urban wastewater nutrients. Aquat. Fish., 1: 64-72 (8 pages).
Oswald, W.J., (1988). Micro-algae and wastewater treatment. Micro-algal Biotech. Cambridge University Press, 22:305–328 (24 pages).
Patel, A.; Barrington, S.; Lefsrud, M., (2012). Microalgae for phosphorus removal and biomass production: a six species screen for dual purpose organisms. GCB Bioenergy, 4(5):485-495(11pages).
Rasoulamini, S.; Montazeri-Najafabady, N.; Mobasher, M.A.; Hoseini-Alhashemi, S.; Ghasemi, Y., (2011). Chlorella sp., a new strain with highly saturated fatty acids for biodiesel production inbubble-column photobioreactor. Appl. Energy, 88 (10): 3354–3356(3 pages).
Rezaei, A.; Sayadi, M.H., (2015). Long-term evolution of the composition of surface water from the River Gharasoo, Iran: a case study using multivariate statistical techniques. Environ. Geochem. Health., 37(2): 251-261(11 pages).
Sayadi, M. H.; Kargar, R.; Doosti, M.R.; Salehi, H., (2012). Hybrid constructed wetlands for wastewater treatment: A worldwide review. Proc. Int. Acad. Eco. Environ. Sci.,2(4): 204-222 (18 pages).
Sayadi, M.H.; Rezaei, A.; Rezaei, M.R.; Nourozi, K., (2014). Multivariate statistical analysis of surface water chemistry: A case study of Gharasoo River, Iran. Proc. Int. Acad. Eco. Environ. Sci., 4(3):114-122 (8 pages).
Shabani, M.; Sayadi M.H.; Rezaei M.R., (2016) CO2 bio-sequestration by Chlorella vulgaris and Spirulina platensis in response to different levels of salinity and CO2. Proc. Int. Acad. Eco. Environ. Sci., 6(2): 53-61(8 pages).
Shahriari Moghadam, M., Safaei, N., Ebrahimipour, G.H., (2016). Optimization of phenol biodegradation by efficient bacteria isolated from petrochemical effluents. Global J. Environ. Sci. Manage., 2 (3): 249-256 (7 pages).
Suva, F. (1999). Technical guidance on pearl hatchery development in the Kingdom of Tonga. FAO. GCP/RAS/116/JPN.
Tam, N.F. Y.; Wong, Y.S., (1994). Effect of immobilized microalgal bead concentrations on wastewater nutrient removal. Environ. Poll.,107: 145-151(7 pages).
Tang, D.; Han, W.; Li, P.; Miao, X.; Zhong, J., (2011). CO2 biofixation and fatty acid composition of Scenedesmus obliquus and Chlorella pyrenoidosa in response to different CO2 levels. Biores. Tech.,102: 3071–3076 (7 pages).
Wang, B.; Lan, C. Q., (2011). Biomass production and nitrogen and phosphorus removal by the green alga Neochloris oleoabundans in simulated wastewater and secondary municipal wastewater effluent. Biores. Tech.,102(10), 5639-5644 (5 pages).
Yang, J.; Xu, M.; Zhang, X.Z.; Hu, M., (2011). Sommerfeld and Y.S. Chen: Life-cycle analysis on biodiesel production from microalgae: Water footprint and nutrients balance. Biores. Tech., 102, 159-165 (6 pages).
Zamani, N.; Nowshadi, M.; Amin, S.; Ghasemi, Y.; Niyazi, A., (2010). Removal of nitrogen-nitrate and ortho phosphate from wastewater using microalgae biotechnology. The second International Symposium on Environmental Engineering. Tehran, Iran 18-20 February.
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