1Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan
2California Department of Food and Agriculture, 3288 Meadowview Road, Sacramento, California, USA
Disastrous effect of nickel on spinach was discussed by number of authors but the effect of amendments like biochar with nickel on Spinacea oleraceaL. is not still discussed by any author of the world because biochar was used as soil amendments which play a vital role in reducing mobilization and uptake of nickel by spinach plants. As nickel contaminated plants are very harmful for the consumption by living organisms. Nickel can be gathered in agronomic soils by anthropogenic actions such as Ni-Cd batteries. In this study, the growth, physiological, photosynthetic and biochemical responses of Spinacia oleracea grown in Ni-spiked soil (0, 25, 50 and 100 mg Ni/Kg soil) at three levels of cotton-sticks-derived biochar “CSB” (0, 3 and 5 %) were evaluated. The results exposed significant decrease in growth, photosynthetic, physiological, and biochemical traits of S. oleracea when grown in Ni-polluted soil. However, this decrease was less pronounced in CSB amended soil. A steady rise in the MDA (0.66 µg/g to 2.08 µg/g), ascorbic acid (1.24 mg/g to 1.57 mg/g)and sugar concentrations (1.73 mg/g to 2.16 mg/g)was observed with increased concentration of Ni. The increasing percentages of CSB from 3 % to 5 % decreased Ni concentrations in root and shoot of experimental plant. Higher production of chlorophyll, amino acids and protein with CSB amendment looked like alleviation in Ni toxicity. Therefore, it is concluded that, Ni toxicity and availability to the plants can be reduced by CSB amendments.
Ahluwalia, Sarabjeet, S. and Dinesh, G. (2007). Microbial and plant derived biomass for removal of heavy metals from wastewater. Bioresource Tech., 98: 2243-2257.
Arnon, D.I. (1949). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta Vulgaris. Plant Physiol., 24: 1-15.
Asai, H., Samson, B., Stephan, H. (2009). Biochar amendment techniques for upland rice production in Northern Laos1. Soil physical properties, leaf SPAD and grain yield. Field Crop Res., 111: 81-84.
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
Cakmak, I., Horst, W.J. (1991). Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol. Plant., 83: 463-468.
Cempel, M., and Nikel, G. (2006). Nickel: a review of its sources and environmental toxicology. Polish J. Environ. Stud., 15: 375-382.
Chaneva, G., Parvanova, P., Tzvetkova, N. and Uzunova, A. (2009). Photosynthetic response of maize plants against cadmium and paraquat impact. Water, Air, Soil Pollut., 208: 287-293.
Demiral, T. and Türkan, I. (2005). Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environ. Exp. Bot., 53: 247-257.
Dicoteau, D. R. (2000). Vegetable crops. New Jersey: Prentice Hall.
Downie, A., Crosky, A., and Munroe, P. (2009). Physical properties of biochar. Biochar for environmental management: Sci.Tech., 13-32.
Gajewska, E., Sklodowska, M., Slaba, M. and Mazur, J. (2006). Effect of nickel on antioxidative enzyme activities, proline and chlorophyll contents in wheat shoots. Plant Biol., 50: 653-659.
Gonnelli, C., Galardi, F., Gabbrielli, R. (2001). Nickel and copper tolerance in three Tuscan populations of Silene paradoxa. Physiol. Plant.,113: 507-514.
Hossain, M.K., Strezov, V., Chan, K.Y. and Nelson, P.F. (2010). Agronomic properties of wastewater sludge biochar and bioavailability of metals in production of cherry tomato (Lycopersicon esculentum). Chemosphere, 78: 1167-1171.
Inouhe, M. (2005). Phytochelatins. Brazilian Journal of Plant Physiology, 17: 65-78.
Jha, P., Biswas, A. K., Lakaria, B. L. and Rao, A. S. (2010). Biochar in agriculture - prospects and related implications. Curr. Sci., 99: 1218-1225.
Keller, T. and Schwager, H. (1977). Air pollution and ascorbic acid. Forest Pathol., 7: 338-350.
Krupa, Z. and Baszynski, T. (1995). Some aspects of heavy metals toxicity towards photosynthetic apparatus-direct and indi- rect effects on light and dark reactions. Acta Physiol. Plant., 17: 177-190.
Lucier, G., and Plummer, C. (2004). Vegetable consumption expected to rise in 2004. Vegetables and melons outlook, Electronic Outlook Report from the Economic Research Service, United States Department of Agriculture VGS-302 April 21, 2004.
Machida, Y.J., Machida, J.K., Teer, A.D. (2005). Acute reduction of an origin recognition complex (ORC) subunit in human cells reveals a requirement of ORC for Cdk2 activation. J. Biol. Chem., 280: 27624-27630
Madhava Rao, K.V. and Sresty, T.V. (2000). Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan (L.) Millsp.) in response to Zn and Ni stresses. Plant Sci., 157: 113-128
Mishra, A. and Choudhuri, M.A. (1999). Monitoring or phytotoxicity of lead and mercury from germination and early seedling growth induces two rice cultivars. Water, Air, Soil Pollut., 114: 339-346.
Mizuno, N. (1968). Interaction between iron and nickel and copper and nickel in various plants. Nature, 219: 1271–1272
Molas, J. (1997). Changes in morphological and anatomical structure of cabbage (Brassica oleacera L.) outer leaves and in ultrastructure of their chloroplasts caused by an in vitro excess of nickel. Photosynthetica 34: 513-522.
Monni, S., Uhlig, C., Junttila, O., Hansen, E. and Hynynen, J. (2001). Chemical composition and ecophysiological responses of Empetrum nigrum to above ground element application. Env. Pollut., 112: 417-426.
Morelock, T.E., and Correll, J.C. (2008). Spinach breeding. In: J. Prohens and F. Nuez (eds.), Vegetables I. Springer, New York, pp. 183-212.
Pandey, N. and Sharma, C.P. (2002). Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth and metabolism of cabbage. Plant Sci., 163: 753-758.
Pant, P.P., Tripathi, A.K. and Dwivedi, V. (2011). Effect of heavy metals on some biochemical parameters of Sal (Shorea robusta ) seedling at nursery level , Doon Valley, India. J. Agri. Sci., 2: 45-51.
Rashid, A. (1986). Mapping zinc fertility of soils using indicator plants and soil analyses. PhD Thesis, University of Hawaii, HI.
Salt, D.E. and Krämer, U. (2000). Mechanisms of metal hyperaccumulation in plants. In: Raskin, I. and Ensley, B.D. (eds.), Phytoremediation of toxic metals - Using plants to clean up the environment. Wiley, New York, pp. 231-246.
Satarug, S., Garrett, S. H., Sens, M. A. and Sens, D. A. (2012). Cadmium environmental exposure, and health outcomes. Env. Health Persp., 118: 182-190.
Scott-Fordsmand, J.J. (1997). Toxicity of nickel to soil organisms in Denmark. Reviews Env. Contam. Toxicol., 148: 1-34.
Seregin, I.V. and Ivanov, V.B. (2001). Physiological aspects of cadmium and lead toxic effects on higher plants. Russian Journal of Plant Physiology, 48: 523-544.
Shah, K. and Nongkynrih, J.M. (2007). Metal hyperaccumulation and bioremediation. Bio. Plant., 51: 618-634.
Sharma, A. and Dhiman, A. (2013). Nickel and Cadmium Toxicity in Plants. J. Pharmaceut. Sci. Innov., 2: 20-24.
Somashekaraiah, B.V., Padmaja K. and Prasad, A.R.K. (1992). Phytotoxicity of cadmium ions on germinating seedlings of mung bean (Phaseolus vulgaris): Involvement of lipid peroxides in chlorophyll degradation. Physiol. Plant., 85: 85-89.
Tripathi, B.N. and Guar, J.P. (2006). Physiological behaviour of Scenedesmus sp.during exposure to elevated levels of Cu and Zn and after withdrawal of metal stress. Protoplasma, 229: 1-9.
Uchimiya, M., Cantrell, K.B., Hunt, P.G., Novak, J.M. and Chang, S (2012). Retention of heavy metals in a typic kandiudult amended with different manure-based biochars. J. Env. Qual. 41: 1138-1149.
Younis, U., Qayyum, M.F., Shah, M.H.R., Danish, S., Shahzad, A.N., Mahmood, S. and Malik, S.A. (2015). Growth, survival and heavy metal (Cd and Ni) uptake of spinach (Spinacia oleracea) and fenugreek (Trigonella corniculata) in a biochar-amended sewage-irrigated contaminated soil. J. Plant Nutr. Soil Sci., DOI.1002/jpln.201400325.
Uzoma, K. C., Inoue, M., Andry, H., Fujimaki, H., Zahoor, A. and Nishihara, E. (2011). Effect of cow manure biochar on maize productivity under sandy soil condition. Soil Use Manage., 27: 205-212.
Verheijen F., Jeffery S., Bastos A.C., Van der Velde M. and Diafas I. (2010). Biochar Application to Soils: A Critical Scientific Review of Effects of Soil Properties, Processes and Functions. JRC Scientific and Technical Reports, EUR 24099 - EN, Italy.
Wagner, A. and Kaupenjohann, M. (2014). Suitability of biochars (pyro- and hydrochars) for metal immobilization on former sewage-field soils. Eurpean J. Soil Sci., 65: 139-148.
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